Protein Info for Psest_1251 in Pseudomonas stutzeri RCH2

Annotation: cysteine desulfurase IscS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 98% identical to ISCS_PSEU5: Cysteine desulfurase IscS (iscS) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 98% identity to psa:PST_3042)

MetaCyc: 72% identical to cysteine desulfurase IscS (Escherichia coli K-12 substr. MG1655)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), IscS subfamily" in subsystem Alanine biosynthesis or Thiamin biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

superpathway of L-alanine biosynthesis (4/4 steps found)
superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
L-alanine biosynthesis III (1/1 steps found)
L-cysteine degradation IV (1/1 steps found)
thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
molybdopterin biosynthesis (4/6 steps found)
thiazole component of thiamine diphosphate biosynthesis I (4/6 steps found)
cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
tRNA-uridine 2-thiolation and selenation (bacteria) (5/11 steps found)
[2Fe-2S] iron-sulfur cluster biosynthesis (4/10 steps found)
tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)

KEGG Metabolic Maps

Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GKG7 at UniProt or InterPro

Protein Sequence (404 amino acids)

>Psest_1251 cysteine desulfurase IscS (Pseudomonas stutzeri RCH2)
MKLPIYLDYSATTPVDPRVAAKMIECLTNEGNFGNPASRSHAFGWKAEEAVENARRQVAE
LVNADPREIVWTSGATESDNLAIKGVAHFYASKGKHIVTTKIEHKAVLDTTRQLEREGFE
VTYIEPGEDGIVTPAMVEAALRDDTILVSVMHVNNEIGTINDITAIGELTRSRGILFHVD
AAQSTGKVEIDLEKMKVDLMSFSAHKTYGPKGVGALYVRRKPRVRLEAQMHGGGHERGMR
SGTLATHQLVGMGEAFRIAKQEMVQENEHIRALRDRFYKQVEHLEELYVNGSMTARVPHN
LNLSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRF
TTEEEIDYAAQKVCEAVTKLRELSPLWDMFKDGVDISKVEWQAH