Protein Info for Psest_1232 in Pseudomonas stutzeri RCH2

Annotation: valyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 944 TIGR00422: valine--tRNA ligase" amino acids 1 to 924 (924 residues), 1196.6 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 622 (609 residues), 526.4 bits, see alignment E=1.2e-161 PF09334: tRNA-synt_1g" amino acids 38 to 90 (53 residues), 20.6 bits, see alignment 4.4e-08 amino acids 359 to 568 (210 residues), 26.6 bits, see alignment E=6.5e-10 PF13603: tRNA-synt_1_2" amino acids 244 to 300 (57 residues), 30.2 bits, see alignment 8e-11 PF08264: Anticodon_1" amino acids 665 to 818 (154 residues), 146.1 bits, see alignment E=1.9e-46 PF10458: Val_tRNA-synt_C" amino acids 879 to 941 (63 residues), 70.9 bits, see alignment 2.1e-23

Best Hits

Swiss-Prot: 66% identical to SYV_ALCBS: Valine--tRNA ligase (valS) from Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 66% identity to abo:ABO_0492)

MetaCyc: 62% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIF2 at UniProt or InterPro

Protein Sequence (944 amino acids)

>Psest_1232 valyl-tRNA synthetase (Pseudomonas stutzeri RCH2)
MDKTYQPHAIETSWYQTWESNNYFAPKGSGEPYTIMIPPPNVTGSLHMGHGFNNAIMDAL
IRWRRMQGRNTLWQPGTDHAGIATQMVVERQLGAQGVSRHDLGREKFLEKVWQWKEESGG
TITRQIRRLGSSVDWSRERFTMDDGLSEAVKEAFVRLHEDGLIYRGKRLVNWDTKLHTAI
SDLEVENHDEKGYLWHLRYPLADGCKTADGLDYLVVATTRPETMLGDAAIAVHPEDERYK
SLIGRHVMLPLVNRLIPIVADDYVDLEFGTGCVKITPAHDFNDYEVGKRHHLPLINIFDQ
NACVLARAQVFNIDGSVNDKLDGSLPDGYAHMDRFDARKAIVAEFEAMSLLEKIDDHALK
VPRGDRSGTIIEPWLTDQWYVSTKPLAEKAIAAVESGEIEFVPKQYENMYFSWMRDIQDW
CISRQLWWGHRIPAWYDEAGNVYVGRDEVEVRSKYNLCNNVELRQDEDVLDTWFSSGLWT
FSTLGWPQQTDFLKTFHPTDVLVTGFDIIFFWVARMIMLSTHLTGQIPFKTVYVHGLVRD
GQGQKMSKSKGNVLDPLDIVDGITLDELLTKRTSGMMQPKLAEKIAKQTRAEFPEGIASY
GTDALRFTFCSLASTGRDIKFDMGRVEGYRNFCNKIWNAANFVFENTEGKDTGANDEPVE
LSSVDRWIISALQRTEAEVTRQLEAFRFDLAAQALYEFIWDEYCAWYLELVKPLLWDETA
SAERQRGTRRALVRVLETALRLAHPFMPFITEEIWQRVAPLAGKSGPTLMLQPWPEFNPE
RIDEAAEGDIEWVKAFMLGIRQIRGEMNISMAKRIDVVLGNASAEDQRRLADNEPLLKKL
AKLESVRLLGAGEEAPLSAIALVGDMQVLVPMAGLIDKDAELARLDKEIARLDGEVKRVG
GKLSNAGFVDKAPAEVIDKERAKLAEAEQAKARLQEQRDRIATL