Protein Info for Psest_1208 in Pseudomonas stutzeri RCH2

Annotation: guanine deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02967: guanine deaminase
  • amino acids 28 to 427 (400 residues), 632.3 bits, E=1.7e-194
• PF01979: Amidohydro_1
  • amino acids 69 to 427 (359 residues), 209.7 bits, E=7.6e-66
• PF07969: Amidohydro_3
  • amino acids 260 to 430 (171 residues), 51 bits, E=1.7e-17

Best Hits

Swiss-Prot: 60% identical to GUAD_ECOLI: Guanine deaminase (guaD) from Escherichia coli (strain K12)

KEGG orthology group: K01487, guanine deaminase [EC: 3.5.4.3] (inferred from 98% identity to psa:PST_3084)

MetaCyc: 60% identical to guanine deaminase (Escherichia coli K-12 substr. MG1655)
Guanine deaminase. [EC: 3.5.4.3]

Predicted SEED Role

"Guanine deaminase (EC 3.5.4.3)" in subsystem Purine Utilization or Purine conversions (EC 3.5.4.3)

MetaCyc Pathways

• guanosine nucleotides degradation II (3/4 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• purine nucleotides degradation II (aerobic) (8/11 steps found)
• drosopterin and aurodrosopterin biosynthesis (5/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• superpathway of guanosine nucleotides degradation (plants) (3/6 steps found)
• purine nucleotides degradation I (plants) (7/12 steps found)
• superpathway of purines degradation in plants (11/18 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.3

Use Curated BLAST to search for 3.5.4.3

Sequence Analysis Tools

See L0GJ04 at UniProt or InterPro

Protein Sequence (435 amino acids)

>Psest_1208 guanine deaminase (Pseudomonas stutzeri RCH2)
MPSTKAYRAALLHCLADPREVGIEQSHEYFEDGLLVVEDGKVARIGHATELLPTLAAGTE
VVEYPDALITPGFVDTHIHYPQVGVIGSYGAQLLDWLETYTFPNESRFSDMAHAREQAEL
FLGELLRNGTTTALVFGTVHKQSVDAFFEACEKRNLRMIAGKVLMDRNAPEFLTDTAESG
YADSRELIERWHGKGRLHYAVTPRFAPTSTPEQLTLAGKLFAEFPDLYMHTHISENKQEV
EWVKELFPERTGYLDVYDHHGLIGPRSVFAHGVHLCDDECKRLGETGSAVSFCPTSNLFL
GSGLFDLAKVEGFGVRVGLGTDVGGGTSFSQLASLNEAYKVLQLQGQKLDAFKALYLATL
GGARALYLDDRIGNLQPGKDADFVVLDYKATPLIDYRLSQATTLQEKLFALMILGDDRAV
KETYAAGASVHRKVC