Protein Info for Psest_1153 in Pseudomonas stutzeri RCH2

Annotation: Cation/multidrug efflux pump

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1018 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 334 to 352 (19 residues), see Phobius details amino acids 359 to 379 (21 residues), see Phobius details amino acids 385 to 409 (25 residues), see Phobius details amino acids 430 to 450 (21 residues), see Phobius details amino acids 462 to 481 (20 residues), see Phobius details amino acids 525 to 544 (20 residues), see Phobius details amino acids 845 to 865 (21 residues), see Phobius details amino acids 872 to 892 (21 residues), see Phobius details amino acids 899 to 922 (24 residues), see Phobius details amino acids 945 to 970 (26 residues), see Phobius details amino acids 976 to 999 (24 residues), see Phobius details PF00873: ACR_tran" amino acids 6 to 1002 (997 residues), 924.4 bits, see alignment E=1.2e-281 PF03176: MMPL" amino acids 294 to 486 (193 residues), 43.9 bits, see alignment E=2.3e-15 amino acids 817 to 1003 (187 residues), 29.3 bits, see alignment E=6.8e-11

Best Hits

Swiss-Prot: 44% identical to Y895_HAEIN: Uncharacterized transporter HI_0895 (HI_0895) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 97% identity to psa:PST_3143)

Predicted SEED Role

"RND multidrug efflux transporter; Acriflavin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIV5 at UniProt or InterPro

Protein Sequence (1018 amino acids)

>Psest_1153 Cation/multidrug efflux pump (Pseudomonas stutzeri RCH2)
MAFTDPFIRRPVLASVISLLIVLLGMQAFNSLTIRQYPQMESALITVTTAYPGANAETIQ
GYITQPLQQSLASAEGVDYMTSVSQQNASVISVYARIGADTDRLYTELLSQANSVKNQLP
QAAEDPVLNKQAADSTALMYISFYSDQLSNPQITDYLSRVIQPKLATLPGMAEAEILGNQ
VFAMRLWLDPVKLAAYGVSAGDVNEAVRKYNFLSAAGEVKGQYVVTSINADTELKTPEAF
AAIPLKTQGDSRVLLGDVARVEMGAESYNSISSFDGIPSVYIGIKGTPSANPLDVIKEVR
AIMPQIEAQLPPGLKASIAYDATEFIQASIDEVVKTLAEAAVIVIVVVFLFLGSLRTVLI
PVVTIPLSMIGVMFFMQAMGYSINLLTLLAMVLAIGLVVDDAIVVVENIHRHIEQGKSPF
QAAIDGAREIAMPVVTMTITLAAVYAPIGFLQGLTGALFQEFALTLAGAVLISGVVALTL
SPMMCSKLLRHEENPSGFAHRLDELFERLKQRYQRALHGTLNTRPVVLVFAVLVLALIPA
LLMFTESELAPEEDQGIVFMMASAPKTANLDYLNAYTDQFLEIFKSFPEYYSWFQINGFD
GVQSGIGGFLLKPWDERERSQMEILPEVQAKLDRLPGLQIFGFNLPSLPGTGEGLPFQFV
INTANDYETLLQVTERIRKRAEESGKFAFLDVDLAFDKPEIVVEIDREKAAQMGVSMEEL
GLTLSTLLGEGEINRFTIEGRSYKVIAQVERAYRDNPDWLSNYYVRNGQGQMLPLSTLIK
VRDRARPTQLKQFQQLNAAMIQGFPIVSMGEAIDTLQTIAREEAPDGFGFDYAGASRQYI
QEGNALYMTFALALAVIFLVLAAQFESFRDPLVILVTVPLSVCGALVPLFLGLSSMNIYT
QVGLVTLIGLISKHGIMIVEFANQLRREQGLSRREAVEEAAAIRLRPVLMTTAATVFGMV
PLIIASGAGAVSRFDIGLVIATGMSVGTLFTLFVLPAVYDLVAQPDRSPSEHAGIAVS