Protein Info for Psest_0011 in Pseudomonas stutzeri RCH2

Annotation: glycyl-tRNA synthetase, tetrameric type, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 315 TIGR00388: glycine--tRNA ligase, alpha subunit" amino acids 10 to 299 (290 residues), 528.2 bits, see alignment E=2.7e-163 PF02091: tRNA-synt_2e" amino acids 11 to 290 (280 residues), 501 bits, see alignment E=4.3e-155

Best Hits

Swiss-Prot: 90% identical to SYGA_PSEAB: Glycine--tRNA ligase alpha subunit (glyQ) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K01878, glycyl-tRNA synthetase alpha chain [EC: 6.1.1.14] (inferred from 93% identity to avn:Avin_00110)

MetaCyc: 73% identical to glycine--tRNA ligase subunit alpha (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase alpha chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GH29 at UniProt or InterPro

Protein Sequence (315 amino acids)

>Psest_0011 glycyl-tRNA synthetase, tetrameric type, alpha subunit (Pseudomonas stutzeri RCH2)
MSQTTPAVRTFQDLILALQSYWAEQGCVVLQPYDMEMGAGTFHTATFLRAIGPETWNAAY
VQPSRRPADGRYGENPNRLQHYYQFQVVLKPNPDNIQDLYLESLRRIGVDTSVHDVRFVE
DNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGVECYPVTGEITYGLERLAMYLQGVD
SVYDLIWADGPFGTVTYGDVFHQNEVEQSTYNFEHANVPKLFELFDFYESEANRLMAAEL
PLPAYEMVVKASHTFNLLDARRAISVTARQQYILRVRSLARSIAQSYLLARAKLGFPMAT
PELRDEVLAKLEAAE