Protein Info for Psest_1129 in Pseudomonas stutzeri RCH2

Annotation: preprotein translocase, SecA subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 918 PF07517: SecA_DEAD" amino acids 12 to 408 (397 residues), 441 bits, see alignment E=5.7e-136 TIGR00963: preprotein translocase, SecA subunit" amino acids 34 to 824 (791 residues), 1112.7 bits, see alignment E=0 PF00270: DEAD" amino acids 105 to 222 (118 residues), 25.2 bits, see alignment E=3.6e-09 PF01043: SecA_PP_bind" amino acids 238 to 364 (127 residues), 118.3 bits, see alignment E=7.6e-38 PF21090: P-loop_SecA" amino acids 423 to 620 (198 residues), 309.1 bits, see alignment E=4.2e-96 PF07516: SecA_SW" amino acids 623 to 836 (214 residues), 234.4 bits, see alignment E=3.9e-73 PF02810: SEC-C" amino acids 898 to 915 (18 residues), 40.5 bits, see alignment (E = 5.8e-14)

Best Hits

Swiss-Prot: 86% identical to SECA_AZOVD: Protein translocase subunit SecA (secA) from Azotobacter vinelandii (strain DJ / ATCC BAA-1303)

KEGG orthology group: K03070, preprotein translocase subunit SecA (inferred from 86% identity to avn:Avin_13340)

Predicted SEED Role

"Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GG25 at UniProt or InterPro

Protein Sequence (918 amino acids)

>Psest_1129 preprotein translocase, SecA subunit (Pseudomonas stutzeri RCH2)
MEVFVDMFAPLLKKLFGSKNEREVKRMLKAVQSVNALEEQMLSLSDEQLRSKTEEFKARL
EKGETLDQILPEAFSVCREAGKRVMGMRHFDVQLIGGMTLHEGRIAEMRTGEGKTLVATL
AVYLNALAGKGVHVVTVNDYLARRDANWMRPLYEFLGLSVGIVTPFQPPEEKRAAYAADI
TYGTNNEFGFDYLRDNMAFSLQEKNQRELNFAVIDEVDSILIDEARTPLIISGQAEDSSK
LYQQINQLIPRLTQHIEEEEGVVTQEGHFSIDEKTRQVELNEAGHQYIEELLTQAGLLAE
GESLYSAHNLGLLTHVYAGLRAHKLFHRNVEYIVQNNQVLLIDEHTGRTMPGRRLSEGLH
QAIEAKEGLQIQPESQTLASTTFQNYFRLYKKLSGMTGTADTEAFEFQQIYNLPVVVIPT
NRPLARKDFNDLVYLTQEEKFAAIIADIKECREQGRPVLVGTATIESSEYVSRLLEKEGF
EHKVLNAKHHDKEAEIIAQAGRPGAVTIATNMAGRGTDILLGGNWEVEVAALENPTEEQV
AQIKADWQKRHQQVLEAGGLHVIASERHESRRIDNQLRGRAGRQGDPGSSRFYLSLEDSL
MRIFASDRVKNFMKALGMESGEAIEHRMVTNAIEKAQRKVEGRNFDMRKQLLEYDDVANE
QRKVIYHMRNSLLAADEIGQTIAEFRQEALDAAISAHIPPQSLPEQWDIPGLEAVLYSDF
GTRLPVQQWLDEDEKLYEETLRERILEALLAAYNEKEDLAGVEALRSFEKQIVLRVLDDL
WKDHLSTMDHLRHGIHLRGYAQKNPKQEYKRESFTLFQDLLESIKRDSIRVLSHVQVRRE
DPAEEEARLRREAEELAKRMQFQHAEVSALDQPEEEPEVEGQADVAATPVRTEPKIGRNE
PCPCGSGKKYKHCHGQVQ