Protein Info for Psest_1104 in Pseudomonas stutzeri RCH2

Annotation: peptidyl-tRNA hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 86% identical to PTH_PSEA7: Peptidyl-tRNA hydrolase (pth) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K01056, peptidyl-tRNA hydrolase, PTH1 family [EC: 3.1.1.29] (inferred from 86% identity to pap:PSPA7_5322)

MetaCyc: 59% identical to peptidyl-tRNA hydrolase (Escherichia coli K-12 substr. MG1655)
Aminoacyl-tRNA hydrolase. [EC: 3.1.1.29]

Predicted SEED Role

"Peptidyl-tRNA hydrolase (EC 3.1.1.29)" (EC 3.1.1.29)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJT2 at UniProt or InterPro

Protein Sequence (194 amino acids)

>Psest_1104 peptidyl-tRNA hydrolase (Pseudomonas stutzeri RCH2)
MTAVKLIVGLGNPGPEYDQTRHNAGALFVERLAAREGVALSNDRKYFGLVGKFSHQGEDI
RLLIPTTYMNRSGQAVAALAGFFRIPPESILVAHDELDMPPGVAKLKQGGGHGGHNGLRD
IIAKLGNQNSFHRLRLGIGHPGHASLVSGYVLGRAPQAEREKLDASIDFALDVVPEILTG
DWTRAMQRLHSQKA