Protein Info for PGA1_c10810 in Phaeobacter inhibens DSM 17395

Annotation: amidophosphoribosyltransferase PurF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01134: amidophosphoribosyltransferase
  • amino acids 19 to 470 (452 residues), 493.9 bits, E=2.9e-152
• PF13522: GATase_6
  • amino acids 84 to 216 (133 residues), 70.4 bits, E=1.6e-23
• PF13537: GATase_7
  • amino acids 105 to 220 (116 residues), 72.7 bits, E=2.9e-24

Best Hits

Swiss-Prot: 55% identical to PUR1_RHIEC: Amidophosphoribosyltransferase (purF) from Rhizobium etli (strain CFN 42 / ATCC 51251)

KEGG orthology group: K00764, amidophosphoribosyltransferase [EC: 2.4.2.14] (inferred from 90% identity to sil:SPO2677)

Predicted SEED Role

"Amidophosphoribosyltransferase (EC 2.4.2.14)" in subsystem De Novo Purine Biosynthesis (EC 2.4.2.14)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (5/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (4/5 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.14

Sequence Analysis Tools

See I7EY42 at UniProt or InterPro

Protein Sequence (488 amino acids)

>PGA1_c10810 amidophosphoribosyltransferase PurF (Phaeobacter inhibens DSM 17395)
MPQMPPAHPFDDDKLREECGIFGVVGVQDASNFVALGLHALQHRGQEAGGIVTHDPVEGF
QSARRFGYVRDNFTSQDVMSTLPGPIGIGHVRYSTAGTKGQTAIRDVQPFFGEFAMGGAA
IAHNGNITNANALRKELIERGSIFQSSSDSECIIHLMARSLQRNIPERMEDALRRVEGAF
SVVAMTRTKLIGVRDPLGVRPLVLGKIGDGYALSSETCALDIIGAEFLREIEPGEMVVIT
DKGVESHFPFRRQPSKFCIFEHVYFSRPDSILGGRSVYETREAIGRELAKENPVEADLVC
PVPDSGTPAAIGYSLQSGIPYAMGIIRNQYMGRTFIEPTEQIRNMGVRLKLNVNRALIQG
KRVILVDDSVVRGTTSRKIKEMILDAGASEVHFRIASPPTAWPCFYGVDTPERDKLLAAT
MTEDEMAKHLAVDSLKFISLDGLYRAVGQDQGRNGACPQYCDACFSGEYPVVPTDMVERG
FEMKPAAE