Protein Info for Psest_1084 in Pseudomonas stutzeri RCH2

Updated annotation (from data): Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)
Rationale: Specifically important for utilizing L-Leucine. Automated validation from mutant phenotype: the predicted function (1.3.8.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Acyl-CoA dehydrogenases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 PF02771: Acyl-CoA_dh_N" amino acids 12 to 123 (112 residues), 143.7 bits, see alignment E=6.2e-46 PF02770: Acyl-CoA_dh_M" amino acids 127 to 222 (96 residues), 93.5 bits, see alignment E=1.5e-30 PF00441: Acyl-CoA_dh_1" amino acids 234 to 382 (149 residues), 146.1 bits, see alignment E=1.8e-46 PF08028: Acyl-CoA_dh_2" amino acids 256 to 365 (110 residues), 48.4 bits, see alignment E=2.4e-16

Best Hits

Swiss-Prot: 66% identical to IVD_SOLTU: Isovaleryl-CoA dehydrogenase, mitochondrial (IVD) from Solanum tuberosum

KEGG orthology group: K00253, isovaleryl-CoA dehydrogenase [EC: 1.3.99.10] (inferred from 98% identity to psa:PST_3213)

MetaCyc: 87% identical to isovaleryl-CoA dehydrogenase subunit (Pseudomonas aeruginosa PAO1)
RXN0-2301 [EC: 1.3.8.4]

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)" (EC 1.3.8.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.4

Use Curated BLAST to search for 1.3.8.4 or 1.3.99.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJR4 at UniProt or InterPro

Protein Sequence (387 amino acids)

>Psest_1084 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (Pseudomonas stutzeri RCH2)
MNYSSLNFALGETIDMLREQVQAFVAAEIAPRAEAIDQENLFPADMWRKFGEMGLLGVTV
SEEYGGAGLGYLAHVVAMEEISRGSASVALSYGAHSNLCVNQINRNGNPEQKARYLPKLI
SGEHVGALAMSEPNAGSDVVSMKLRAEKRGDRYVLNGSKTWITNGPDANTYVIYAKTDLD
KGAHGITAFIVERDWKGFSRGNKFDKLGMRGSNTCELFFDDVEVPQENVLGAENGGVKVL
MSGLDYERVVLAGGPTGIMQSCLDVVVPYIHDRKQFGQSIGEFQFIQGKVADMYTQLNAS
RAYLYAVAQACDRGETTRKDAAGVILYTAENATQMALQAIQILGGNGYINEFPTGRLLRD
AKLYEIGAGTSEIRRMLIGRELFNESR