Protein Info for Psest_1079 in Pseudomonas stutzeri RCH2

Annotation: Isopropylmalate/homocitrate/citramalate synthases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 79% identical to LIUE_PSEAE: 3-hydroxy-3-isohexenylglutaryl-CoA/hydroxy-methylglutaryl-CoA lyase (liuE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01640, hydroxymethylglutaryl-CoA lyase [EC: 4.1.3.4] (inferred from 98% identity to psa:PST_3217)

MetaCyc: 79% identical to hydroxymethylglutaryl-CoA lyase subunit (Pseudomonas aeruginosa PAO1)
Hydroxymethylglutaryl-CoA lyase. [EC: 4.1.3.4]; 3-hydroxy-3-isohexenylglutaryl-CoA lyase. [EC: 4.1.3.4, 4.1.3.26]

Predicted SEED Role

"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)

MetaCyc Pathways

L-leucine degradation I (5/6 steps found)
mevalonate degradation (1/2 steps found)
ketogenesis (3/5 steps found)
cis-geranyl-CoA degradation (2/9 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.4

Use Curated BLAST to search for 4.1.3.26 or 4.1.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJR0 at UniProt or InterPro

Protein Sequence (299 amino acids)

>Psest_1079 Isopropylmalate/homocitrate/citramalate synthases (Pseudomonas stutzeri RCH2)
MSLPKQVRLVEVGPRDGLQNEQQPISVADKVRLVDDLSAAGLSYIEVGSFVSPKWVPQMA
GSAEVFAQIQRKAGVTYAALTPNMKGLEAAIEAGVKEVAVFGAASEAFSQKNINCSIAES
LARFAPLMEAAREQDIRVRGYVSCVLGCPYEGEVAPAKVAEVARELYAMGCYEVSLGDTI
GTGTPGKTRSLFDTVAREVPRERLAGHFHDTYGQALPNIYASLLEGIAVFDSSVAGLGGC
PYAKGASGNVASEDVLYMFNGLDIATGIDLDALIGAGQRISQLLGRANGSRVARARQAG