Protein Info for PGA1_c01060 in Phaeobacter inhibens DSM 17395

Annotation: ribonuclease 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 228 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR02191: ribonuclease III" amino acids 10 to 223 (214 residues), 227.6 bits, see alignment E=6.7e-72 PF14622: Ribonucleas_3_3" amino acids 22 to 142 (121 residues), 109.3 bits, see alignment E=2.3e-35 PF00636: Ribonuclease_3" amino acids 42 to 132 (91 residues), 62.3 bits, see alignment E=1e-20 PF00035: dsrm" amino acids 158 to 222 (65 residues), 54.4 bits, see alignment E=2.4e-18

Best Hits

Swiss-Prot: 76% identical to RNC_RUEPO: Ribonuclease 3 (rnc) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)

KEGG orthology group: K03685, ribonuclease III [EC: 3.1.26.3] (inferred from 79% identity to sit:TM1040_2559)

MetaCyc: 40% identical to RNase III (Escherichia coli K-12 substr. MG1655)
Ribonuclease III. [EC: 3.1.26.3]

Predicted SEED Role

"Ribonuclease III (EC 3.1.26.3)" in subsystem RNA processing and degradation, bacterial or Two cell division clusters relating to chromosome partitioning (EC 3.1.26.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.3

Use Curated BLAST to search for 3.1.26.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7ET73 at UniProt or InterPro

Protein Sequence (228 amino acids)

>PGA1_c01060 ribonuclease 3 (Phaeobacter inhibens DSM 17395)
MKLSKDMRAFETRIGYSFSQPALLVRALTHASVSSPNRQDNQRLEFLGDRVLGLVMATAL
LDTDKAATEGQLAPRFNALVRKETCADVARDIDLGAVLKLGRSETMSGGRRKLALLGDAM
EAVIAAVYKDGGFDAAQELILRLWGNRIGRVEADARDPKTTLQEFAQARGEQPPAYVLVE
RTGPDHQPEFTISVQLQDGTEGRATAGSKRQAEQAAAKFLLARLEQEK