Protein Info for Psest_1069 in Pseudomonas stutzeri RCH2

Annotation: Protease II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 609 signal peptide" amino acids 1 to 70 (70 residues), see Phobius details PF07519: Tannase" amino acids 118 to 602 (485 residues), 367.2 bits, see alignment E=1.5e-113 PF00326: Peptidase_S9" amino acids 198 to 275 (78 residues), 22.9 bits, see alignment E=5.4e-09

Best Hits

KEGG orthology group: K09252, feruloyl esterase [EC: 3.1.1.73] (inferred from 84% identity to pmy:Pmen_4357)

Predicted SEED Role

"tannase precursor"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.73

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJQ2 at UniProt or InterPro

Protein Sequence (609 amino acids)

>Psest_1069 Protease II (Pseudomonas stutzeri RCH2)
MTGHRLPTLPSARWHVIGLSAYAPDPAHRLDNNKKEASMMPIRSNPFTRRALCLTPLALA
LFAAEAVQAATCTELLGHEIPAQAIGLPTSGARVTAASSVAAGGSAPQTFGAYCDLSAEI
RPIDPAAPAIRMRLVLPEQWNRKAMMFGGGGYNGTVPNVAGNVPAGPVDQPTPLGRGYAV
FGSDSGHVADPLSPGAFAWNDEALANYAHDALKKTRDAAVYLIEQRYSAAPERSYFAGGS
TGGREALAVVQQWPTDFNGAIVLYPAYNALALDLQFGRITRALAQPGAFPSLEKRAALLE
AAMQACDGLDGVRDGVISHQAACNAQFDPARAKLNGKPLRCPGGADTSQRCLSDAQIQAL
RVFNTPIRFNFPLASGETHYPGFNTWGTDLGRPGEGVQLVVNRLGLNTLQPSYPMPVHGT
GFAEGAPYHSGFWDEWVRFFVTRNPTFNSLTLDPQNPGPWQARISELSLRQDVNRTDLSA
FARNGGKILMAHGTSDQLVSTRATAEYYERVRRDMGPGKTQRFLRYYEIPGYGHAASTVF
NAAWDSLSALEDWVEQGRAPRRQVVADSIGVPGRTRPLCEYPGWPKYVGKGDVNAASSFV
CVQSRKGTR