Protein Info for Psest_0103 in Pseudomonas stutzeri RCH2

Annotation: triphosphoribosyl-dephospho-CoA synthase MdcB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 292 TIGR03132: triphosphoribosyl-dephospho-CoA synthase MdcB" amino acids 14 to 280 (267 residues), 337.4 bits, see alignment E=3.5e-105 PF01874: CitG" amino acids 18 to 279 (262 residues), 261.4 bits, see alignment E=6.6e-82

Best Hits

Swiss-Prot: 84% identical to MDCB_PSEU5: Probable 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase (mdcB) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K13930, triphosphoribosyl-dephospho-CoA synthase [EC: 2.7.8.25] (inferred from 84% identity to psa:PST_4119)

Predicted SEED Role

"Triphosphoribosyl-dephospho-CoA synthetase (EC 2.7.8.25)" in subsystem Malonate decarboxylase (EC 2.7.8.25)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GFB8 at UniProt or InterPro

Protein Sequence (292 amino acids)

>Psest_0103 triphosphoribosyl-dephospho-CoA synthase MdcB (Pseudomonas stutzeri RCH2)
MSALIARQAQPTLAERLADLAVQALIDEADLSPKPGLVDRRGNGAHSDLHLGLMHASAHA
LWPAFKAMAEAAQEVGEVGQPLRETLGQLGRDGEADMLRVTGGVNTHRGAIWALGLLSAA
AALQADAPDSRGVAATAAALARLSDPAAPVTSDSHGARVCRRYGVLGAREQAQYGFPAVI
EHGLPQLLASRRAGAGEQNARLDALLAIMSSLTDTCVLHRAGLEGLTRMQAGARAVLEAG
GAASLAGRRQLRLLDRDMLALNASPGGAADLLAATLFLDCLAPHAPAPTGSN