Protein Info for Psest_1060 in Pseudomonas stutzeri RCH2

Annotation: trehalose-phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 258 TIGR00685: trehalose-phosphatase" amino acids 13 to 244 (232 residues), 182.2 bits, see alignment E=1.6e-57 PF08282: Hydrolase_3" amino acids 17 to 110 (94 residues), 27.7 bits, see alignment E=2.3e-10 TIGR01484: HAD hydrolase, family IIB" amino acids 17 to 210 (194 residues), 83.1 bits, see alignment E=3e-27 PF02358: Trehalose_PPase" amino acids 18 to 219 (202 residues), 116 bits, see alignment E=1.5e-37

Best Hits

Swiss-Prot: 53% identical to OTSB_SALTY: Trehalose-phosphate phosphatase (otsB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01087, trehalose-phosphatase [EC: 3.1.3.12] (inferred from 96% identity to psa:PST_3236)

Predicted SEED Role

"Trehalose-6-phosphate phosphatase (EC 3.1.3.12)" in subsystem Trehalose Biosynthesis (EC 3.1.3.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIM0 at UniProt or InterPro

Protein Sequence (258 amino acids)

>Psest_1060 trehalose-phosphatase (Pseudomonas stutzeri RCH2)
MNDQNDQPAPEPRRYAFFFDVDGTLAEIQPRPELVFIPPATLAALERLHAGGIPVAVISG
RPLAQLDALLAPLQLPAAGVHGAERRDATGELRNLALDKQALERIQRELQQACSEHPGLH
LENKSVAFALHFRQAPELEEIARTLAEDFAQRYAEVLTLQPGKCVFELKPRGASKGEVIR
AFMQEAPFAGRTPVFLGDDLTDEAGFAAVNALGGRSIKVGDGASEAHERVASVAAVGSWI
EALLNELGVPAEPRTKPD