Protein Info for Psest_0010 in Pseudomonas stutzeri RCH2

Annotation: glycyl-tRNA synthetase, tetrameric type, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 684 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 683 (683 residues), 799.4 bits, see alignment E=2.1e-244 PF02092: tRNA_synt_2f" amino acids 6 to 541 (536 residues), 690.1 bits, see alignment E=2.7e-211 PF05746: DALR_1" amino acids 576 to 670 (95 residues), 35.8 bits, see alignment E=7.6e-13

Best Hits

Swiss-Prot: 96% identical to SYGB_PSEU5: Glycine--tRNA ligase beta subunit (glyS) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 96% identity to psa:PST_0009)

MetaCyc: 51% identical to glycine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GD32 at UniProt or InterPro

Protein Sequence (684 amino acids)

>Psest_0010 glycyl-tRNA synthetase, tetrameric type, beta subunit (Pseudomonas stutzeri RCH2)
MSALDFLVELGTEELPPKALAKLAEAFCAGIEKGLKDAGLSFVKTQAYAAPRRLAVLVEQ
LATQQPDRSINLDGPPMQAAFDADGEPTQAALGFARKCGVDLAEIDRSGPKLKFSRTIEG
QPASQLLPGIVEASLNDLPIPKRMRWAARKEEFVRPTQWLVMLFGEQVIDCEILAQRAGR
ESRGHRFHSPGQVHISKPSSYLEDLRGAHVIADFAERRELIAKRVEQLAAEQNGTAIVPP
ALLDEVTALVEWPVPLVCSFEERFLEVPQEALITTMQDNQKYFCLLDANGKLLPRFITVA
NIESKDPAQIVSGNEKVVRPRLTDAEFFFKQDKKQKLDSFNERLKNVVFQAQLGTVYDKA
ERVSRLAGLIAERTGGDKQRAARAGLLSKCDLATEMVGEFPEMQGIAGYYYALNDGEPED
VALALNEQYMPRGAGGELPSTLTGAAVAVADKLDTLVGIFGIGMLPTGSKDPYALRRAAL
GVLRILIEKRLDLNLVEAVNFAIGQFGTQVKSAGLADQVLEFIFDRLRARYEDEGVDVAA
YLSVRAVQPGSALDFDQRVQAVQAFRTLPEAAALAAANKRVSNLLSKFDAKLPEAVEPRW
FDNATEFSLYSAIQQAEQAVQPLAAARQYREALERLAHLRGPVDAFFEAVLVNAEDASVR
ANRYALLARLRGLFLGVADISALG