Protein Info for EX31_RS22475 in Rahnella sp. WP5

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00529: CusB_dom_1" amino acids 38 to 361 (324 residues), 56.9 bits, see alignment E=3.3e-19 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 40 to 375 (336 residues), 285.1 bits, see alignment E=3e-89 PF16576: HlyD_D23" amino acids 64 to 293 (230 residues), 44.3 bits, see alignment E=2.6e-15 PF13437: HlyD_3" amino acids 175 to 290 (116 residues), 34.2 bits, see alignment E=7.2e-12

Best Hits

Swiss-Prot: 76% identical to ACRA_ECO57: Multidrug efflux pump subunit AcrA (acrA) from Escherichia coli O157:H7

KEGG orthology group: K03585, membrane fusion protein (inferred from 100% identity to rah:Rahaq_3319)

MetaCyc: 76% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"Membrane fusion protein of RND family multidrug efflux pump" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (391 amino acids)

>EX31_RS22475 efflux RND transporter periplasmic adaptor subunit (Rahnella sp. WP5)
MNKNRGLTPLAVVLMLSGSLALTGCNDKEAQQGAPQAPQVGVVTLKTEPLNVTTELPGRT
AAFRIAEVRPQVNGIILKRNFVEGSDIKAGTSLYQIDPATYQAAYDSAKGDLVKAQANAQ
ISRLTVTRYKPLLGTSYISKQDYDTAVATAAQADAAVVSAKAAVESARINLAYTKVTSPI
SGRIGKSSVTEGALVSNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELADGSLKQADG
KAQVKLLMDNGKEYSQPGTLEFSDVTVDETTGSITLRAIFPNPNDSLLPGMFVRARLDEG
VNNNALLVPQQGITRNPRGDATAMVIGADNKVELRTVTTTQAIGDKWVVTDGLKSGDKVI
VTGLQKIKPGVQVTAQEVDQNAAAPATATKS