Protein Info for EX31_RS14590 in Rahnella sp. WP5

Annotation: transcription/translation regulatory transformer protein RfaH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 179 PF02357: NusG" amino acids 20 to 113 (94 residues), 74.1 bits, see alignment E=5.3e-25 TIGR01955: transcription elongation factor/antiterminator RfaH" amino acids 21 to 176 (156 residues), 206.6 bits, see alignment E=1e-65

Best Hits

Swiss-Prot: 70% identical to RFAH_ECOL5: Transcription antitermination protein RfaH (rfaH) from Escherichia coli O6:K15:H31 (strain 536 / UPEC)

KEGG orthology group: K05785, transcriptional antiterminator RfaH (inferred from 100% identity to rah:Rahaq_4154)

Predicted SEED Role

"Transcriptional activator RfaH" in subsystem Transcription factors bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (179 amino acids)

>EX31_RS14590 transcription/translation regulatory transformer protein RfaH (Rahnella sp. WP5)
MSGFWYAAPLNAFMWETMESWYLLYCKRGQLLRAKEHLERQNVNCLTPMITLEKLVRGRR
TAVSEPLFPNYMFIEFDPERIHTTTINATRGVSHFIRFGAKLATVQPDVIKQLMEPPTIE
IRDPDTPYEGDQVVITDGIFAGIEAIYTEPDGEARSLLMLNLINQPVRQSIENTHFQKI