Protein Info for EX31_RS13925 in Rahnella sp. WP5

Annotation: FAD-binding oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1018 PF01565: FAD_binding_4" amino acids 52 to 192 (141 residues), 111.5 bits, see alignment E=4e-36 PF02913: FAD-oxidase_C" amino acids 281 to 539 (259 residues), 210.2 bits, see alignment E=6.5e-66 PF13183: Fer4_8" amino acids 577 to 687 (111 residues), 29.2 bits, see alignment E=1.7e-10

Best Hits

Swiss-Prot: 78% identical to YDIJ_ECOLI: Uncharacterized protein YdiJ (ydiJ) from Escherichia coli (strain K12)

KEGG orthology group: K06911, (no description) (inferred from 63% identity to aha:AHA_2649)

MetaCyc: 78% identical to D-2-hydroxyglutarate dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-14932 [EC: 1.1.99.39]

Predicted SEED Role

"Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.99.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.99.14 or 1.1.99.39

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1018 amino acids)

>EX31_RS13925 FAD-binding oxidoreductase (Rahnella sp. WP5)
MIPQISQAPGVIQLVLDFLEALKSNGFTGDVTTTYADRLTMATDNSVYQLLPDAVLFPIA
TSDVALIARLAGQERFKTLVFTPRGGGTGTNGQSLNRGIVVDMSRHMNRILDINVEQGWV
KVEAGVIKDQLNDYLRPFGFFFSPELSTSNRATLGGMINTDASGQGSLVYGKTSDHVLGV
RAIVLGGDMLDTTAIPTALADKLGEEDSVIGRIYRTVLDSCRDNRALVIEKFPKLNRFLT
GYDLRHVLSDDLQTFNLTRILTGSEGSLAFITEARLNITPIPKVRRLVNIKYDSFNSALR
NAPFMVEAKALSVETVDSKVLNLAREDIVWHSVRDYITDVEGKDMQGLNIVEFAGDDADL
IETQVSALCERLDSLISDKEGGVIGYQICADLAGIERIYNMRKKAVGLLGNAKGQAKPLP
FAEDTCVPPQHLADYIVEFRALLDSHHLSYGMFGHVDAGVLHVRPALDMCDPQQEILLKQ
ISDEVVALTAKYGGLLWGEHGKGFRAEYSPAFFGEALFHELRRIKTVFDPDNRLNPGKIC
APLECDEPMMKVDAPKRGTLDRRIPLEVRQSFRGAMECNGNGLCFNFDVKSPMCPSMKIT
GSRIHSPKGRAGLVREWLRLLSEQGVDPLALENALPEKGMSFRSLIERTRNTWHANKGEY
DFSHEVKEAMSGCLACKACSTQCPIKIDVPGFRSRFLQLYHTRYLRPARDHFVASVESYA
PLMAKAPKVFNFFLRQPWLREMSRKSIGMVDLPLLSSPTLREQLSGHRASKMTLEQLEAI
PSARRGEYVLVVQDPFTSFYDAQVVADFIRLIEKLGLKPVLLPFSPNGKAQHIKGFLTRF
AKTAKKTSLFLNRVSQLGMPMVGVDPALVLCYRDEYKEILGDTRGSFQVQLVHEWLDSWL
AERPAQQQSGESWYLFGHCTESTALPNSGKQWGDIFARYGAKLENVSVGCCGMAGTYGHE
TKNLENSLGIYELSWHTSLQRLPRQRCLATGYSCRSQVKRIEGNGVRHPLQALLEIIP