Protein Info for EX31_RS13545 in Rahnella sp. WP5

Annotation: PTS 2-O-a-mannosyl-D-glycerate transporter subunit IIABC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 642 transmembrane" amino acids 297 to 322 (26 residues), see Phobius details amino acids 340 to 360 (21 residues), see Phobius details amino acids 372 to 398 (27 residues), see Phobius details amino acids 418 to 438 (21 residues), see Phobius details amino acids 450 to 471 (22 residues), see Phobius details amino acids 482 to 501 (20 residues), see Phobius details amino acids 507 to 524 (18 residues), see Phobius details amino acids 536 to 551 (16 residues), see Phobius details amino acids 559 to 582 (24 residues), see Phobius details amino acids 600 to 625 (26 residues), see Phobius details PF00359: PTS_EIIA_2" amino acids 6 to 148 (143 residues), 122.1 bits, see alignment E=1.8e-39 TIGR00848: PTS system, fructose subfamily, IIA component" amino acids 8 to 135 (128 residues), 119.9 bits, see alignment E=1e-38 TIGR00829: PTS system, Fru family, IIB component" amino acids 170 to 251 (82 residues), 78.7 bits, see alignment E=4.9e-26 PF02302: PTS_IIB" amino acids 171 to 262 (92 residues), 68.4 bits, see alignment E=7e-23 TIGR01427: PTS system, Fru family, IIC component" amino acids 284 to 624 (341 residues), 265.6 bits, see alignment E=1.2e-82

Best Hits

Swiss-Prot: 68% identical to MNGA_ECOLI: PTS system 2-O-alpha-mannosyl-D-glycerate-specific EIIABC component (mngA) from Escherichia coli (strain K12)

KEGG orthology group: K11198, PTS system, 2-O-A-mannosyl-D-glycerate-specific IIA component [EC: 2.7.1.69] K11199, PTS system, 2-O-A-mannosyl-D-glycerate-specific IIB component [EC: 2.7.1.69] K11200, PTS system, 2-O-A-mannosyl-D-glycerate-specific IIC component (inferred from 70% identity to avr:B565_2243)

MetaCyc: 68% identical to 2-O-alpha-mannosyl-D-glycerate specific PTS enzyme II (Escherichia coli K-12 substr. MG1655)
RXN0-2522 [EC: 2.7.1.195]

Predicted SEED Role

"PTS system, 2-O-alpha-mannosyl-D-glycerate-specific IIA component / PTS system, 2-O-alpha-mannosyl-D-glycerate-specific IIB component / PTS system, 2-O-alpha-mannosyl-D-glycerate-specific IIC component"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.195 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (642 amino acids)

>EX31_RS13545 PTS 2-O-a-mannosyl-D-glycerate transporter subunit IIABC (Rahnella sp. WP5)
MDLTSLTSPDLLHLNTRFSSREQAIGFLVRQLDQQGILDNAQAFLHAVTERENEGPTALG
KGLAVPHGKSASVRRAAFALATLEHPLLWQGVDGDEPVNMIFLLAIPEAEAGSTHMQLLT
ALTTRLVDDETRAALLAARNAQDVLALLGAEPAPESRDPQALPDDAPLVVCVTACPAGIA
HTYMAAEYLEKAAKKLGIRIHVEKQGASGIEDPITAAQCDRAVACVFAAEVAVKDSQRFD
GIPRVQVPVAEPIRRAEAVLQQALAIQRQPGETVTSAPVKPQKTSIRADLKQALLSGISF
AVPLIVAGGSLLAVTVLIAQLFGWQEIYAQKESWLWMYRQLGGGMLGILMVPVLSAYTAY
SLADKPALTPGFAAGLCANMIGAGFLGGVAGGLIAGYVMRWMKKHVRLSASFNGFLMFYL
YPVLGTLVTGSIMLFIIGEPVAWLNTSLTAWLNGLSGSNALVLGALIAFMCSFDLGGPVN
KAAYAFCLGAMANGVYGPYAIFGATKMVSAFTVTASTLLAPHLFKSFEIETGKSTWLLGL
AGITEGAIPMAIEDPLRVIGSFLAGSVVTGAIVGAAGIGLSTPGAGIFSMFLLHDGGLGG
WLSAAIWLGAALAGTVISTLVLLSWRGYAVRQGHFTVDIPSS