Protein Info for EX31_RS10800 in Rahnella sp. WP5

Annotation: DHA2 family efflux MFS transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 55 to 75 (21 residues), see Phobius details amino acids 82 to 102 (21 residues), see Phobius details amino acids 108 to 130 (23 residues), see Phobius details amino acids 142 to 163 (22 residues), see Phobius details amino acids 169 to 190 (22 residues), see Phobius details amino acids 206 to 225 (20 residues), see Phobius details amino acids 237 to 258 (22 residues), see Phobius details amino acids 277 to 298 (22 residues), see Phobius details amino acids 306 to 330 (25 residues), see Phobius details amino acids 340 to 358 (19 residues), see Phobius details amino acids 373 to 394 (22 residues), see Phobius details amino acids 486 to 511 (26 residues), see Phobius details PF00083: Sugar_tr" amino acids 18 to 196 (179 residues), 48.2 bits, see alignment E=8.2e-17 TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 18 to 508 (491 residues), 432.1 bits, see alignment E=1.4e-133 PF07690: MFS_1" amino acids 22 to 417 (396 residues), 187 bits, see alignment E=4.7e-59

Best Hits

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 100% identity to rah:Rahaq_1956)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (522 amino acids)

>EX31_RS10800 DHA2 family efflux MFS transporter permease subunit (Rahnella sp. WP5)
MNEDRSYWKPKANPWAVAAVVTIAVFMEILDTTIVNVALPHVAGSLSSSYDESTWVLTSY
LVANGIVLPISAFLSRVMGRKTYFLICIGMFTVCSFLCGIATELWQMILFRILQGFFGGG
LQPIQQSVLLDYFKPEDRGKAFGLSSIAIIVAPVVGPTLGGYITDNFSWRWVFLINIPVG
IIALLAIYQLLEDPPWERKAKGKLSIDYIGIGLIALGLGCLQVMMDRGEDDDWFQSGFIV
TFAVLAATGIVGAVYWLLYAKKPVVDLRCMKDKNFAVAGLLMAGMAMILYGSSVVIPQLA
QQQLGYTATLSGLVMSPGAILIVLSIPLVLKLMPVVQTRYIIAFGFFLLAASFIYSATLT
PDVDFETLVLYRSAQSIGLGFLFVPLTTIAFITIPQKMNADAAALFTMFRNVAGSIGISL
ATAGVTERTQAHSAHMAHNMSPLNEQFNQAVSKSAEAIRDFAHVVGDPMQLATVRMYQEM
ISQSRILAYIDVFGYCAIVAVILIPFCFLLSPVKSEGNAGAH