Protein Info for EX31_RS09405 in Rahnella sp. WP5

Annotation: iron uptake system protein EfeO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF13473: Cupredoxin_1" amino acids 16 to 116 (101 residues), 83.6 bits, see alignment E=9.5e-28 PF09375: Peptidase_M75" amino acids 136 to 367 (232 residues), 144.2 bits, see alignment E=6.6e-46

Best Hits

Swiss-Prot: 83% identical to EFEO_YERPA: Iron uptake system component EfeO (efeO) from Yersinia pestis bv. Antiqua (strain Antiqua)

KEGG orthology group: K07224, putative lipoprotein (inferred from 100% identity to rah:Rahaq_2461)

MetaCyc: 72% identical to ferrous iron transport system protein EfeO (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Ferrous iron transport periplasmic protein EfeO, contains peptidase-M75 domain and (frequently) cupredoxin-like domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (375 amino acids)

>EX31_RS09405 iron uptake system protein EfeO (Rahnella sp. WP5)
MTHFRRKALQIALLSLPAFALSANVLAADVRQVKIAVNDKQCEPMALTVPAGKTQFVIHN
NSQKNLEWEILKGVMVVEERENVAPGFTQKMTANLEPGEYDMTCGLLSNPKGKLTVTAEG
TAASGKPDAMALVGPIAEYKVYVTQQVAELVAHTKAFTDAVKKGDLAKAQSLYASTRVYY
ERIEPIAELFSDLDGSIDAREDDFEKKAEDPNFTGFHRLEKILFADKTTKGTDPFADKLY
TDTVDLQKRIADLTFPPSKVVGGAAGLIEEVAASKISGEEDRYSRTDLWDFRANLDGAQK
IVNLLRPLLVKADKPLLDKIDANFKTVDALLDKYKTKDGYENYEKLSDADRNAMKGPITT
LAEDLSKLRGVLGLD