Protein Info for EX31_RS03460 in Rahnella sp. WP5
Annotation: glycerophosphodiester phosphodiesterase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Predicted SEED Role
"Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization (EC 3.1.4.46)
MetaCyc Pathways
- glycerol and glycerophosphodiester degradation (4/4 steps found)
- glycerophosphodiester degradation (2/2 steps found)
- phosphatidylcholine resynthesis via glycerophosphocholine (1/2 steps found)
- phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)
- anandamide biosynthesis I (4/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.1.4.46
Use Curated BLAST to search for 3.1.4.46
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (776 amino acids)
>EX31_RS03460 glycerophosphodiester phosphodiesterase (Rahnella sp. WP5) MFTSQSNVNITYSDTIVITDEAPPANGFKQLTTQTSTQYIPSTEIHSTCNAGQDGQAFSG SVAGYQGSLDIFTAINGKNVQLYGTSIPGIFYAVKMYSKKCPEMGGYVPPSKDWTFLYDV GDDDEKSCLKSDEPYYFDLAFFMNSDYQPREDTIAIQNIPIQEHGNFKLSGTDGDDAQGK ATVETVQFVAELRKNATECPTSATLLNELQNKLAKNNQIVTAQHRGDFNDTQPENSLGAF HLSYDRCRANVETDVRATSDGELVVFHDLNIGKMLEPTYDPIANSGPNVALSSLTLAELQ QKYLVNVATRQPTTYTVPTVNQLIDDYIAYNGQSLIYLETKSSSVIMPTALALYNKSVTY SGSNLLQRIIMKVNMAEYPSPDLWNNALINAGIPAGVTIMIDPVITPNDASKINDLPDTT FACPAGISDSKAVCAVRAWANAPATLAPMVSVLIKDSADFEEVSTKQNEQGSYDAPANFE LSNTVSGTVAEMVAVIKGAGKALEIFSPVPDYMLWKNLNFYTETVYDKNIPQDISVREAF YNNDSSCCYRVLDKLSPSTIAAEKNDYRMNLGWLRDIGANVITADDTDSINTYFSGTGVL DRALTPHVWTPSFYMQSILSWQLGSAVMEDKSLASGKDAYALYNNDDSYAWTYRSDPPIA VMRAGYYAWMSVKRQPDGRVRISPAYNKEQCLRSALSNHSWDYVGWKKDCTSENTLWEMQ PKSGSDDWFSLIDAHHMSLGWAYSGKLYYAYSYGYVYVDKPSTLSDPYYWSFGTAK