Protein Info for EX31_RS01860 in Rahnella sp. WP5

Annotation: type VI secretion system baseplate subunit TssK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 transmembrane" amino acids 187 to 205 (19 residues), see Phobius details amino acids 226 to 243 (18 residues), see Phobius details TIGR03353: type VI secretion protein, VC_A0114 family" amino acids 5 to 443 (439 residues), 403.3 bits, see alignment E=5.9e-125 PF05936: T6SS_VasE" amino acids 18 to 442 (425 residues), 412.7 bits, see alignment E=8.9e-128

Best Hits

KEGG orthology group: K11893, type VI secretion system protein ImpJ (inferred from 76% identity to esa:ESA_pESA3p05493)

Predicted SEED Role

"Uncharacterized protein ImpJ/VasE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (445 amino acids)

>EX31_RS01860 type VI secretion system baseplate subunit TssK (Rahnella sp. WP5)
MKIYRPLWSDGAFLAPQQFQQQARWDAFVSDRVAHMALANPWGVIDATFDIGSLALSRLN
ALRLKVRFPDGTLIDTELADNLPPACDLSAISDRDSVDVVLALPLLSANGGNLDDGQDSE
RPRRWQQEWVTVQELSGHERTELAIQRYAITLRFAHQENSAYLTCPVVRLVRNAQGQWTP
DAEFIPPMLSLSSSPAVLTELGDLIHRLQARRRRLMAMRRESNERMADFAVADVSLFWLL
NALNSAEPVLMELYQSPARHPELFYRELVRLAGSLLTFSLEHKAEDIPLYQHNAPENVFP
PLFTLLDALLEASLPSRMVAIELTHEGQFWTGSLHDSRLRDGADFYLSVRSSIPNHLLQT
QFPLLCKAGSFEDVSDVVNVALNGVVMKALSHVPAAIPLRLENQYFALDLNSAAAQAMLD
AGNCAFYTPGSLGEIKLELFAVLRS