Protein Info for ECOLIN_RS17390 in Escherichia coli Nissle 1917

Annotation: TRAP transporter large permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 transmembrane" amino acids 6 to 36 (31 residues), see Phobius details amino acids 57 to 77 (21 residues), see Phobius details amino acids 106 to 130 (25 residues), see Phobius details amino acids 142 to 169 (28 residues), see Phobius details amino acids 176 to 201 (26 residues), see Phobius details amino acids 222 to 244 (23 residues), see Phobius details amino acids 250 to 267 (18 residues), see Phobius details amino acids 277 to 300 (24 residues), see Phobius details amino acids 321 to 359 (39 residues), see Phobius details amino acids 365 to 392 (28 residues), see Phobius details amino acids 404 to 428 (25 residues), see Phobius details PF06808: DctM" amino acids 13 to 424 (412 residues), 418.3 bits, see alignment E=3.3e-129 TIGR00786: TRAP transporter, DctM subunit" amino acids 23 to 429 (407 residues), 513.4 bits, see alignment E=1.9e-158

Best Hits

Swiss-Prot: 90% identical to YGIK_SALTY: Uncharacterized protein YgiK (ygiK) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 98% identity to ecp:ECP_3105)

MetaCyc: 37% identical to 2,3-diketo-L-gulonate:Na+ symporter - membrane subunit (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-235

Predicted SEED Role

"TRAP-type C4-dicarboxylate transport system, large permease component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>ECOLIN_RS17390 TRAP transporter large permease subunit (Escherichia coli Nissle 1917)
MDFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPPDAAISVISQKMTVGLD
GFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLAHCNILANTLFGAISGSAV
ASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPPSNVLIVYSLASGGTSVAALF
LAGYLPGILTAVALMFVAALYARRNHYPVAERINFRQFLQVFRESIPSLMLIFIIIGGII
AGVFTPTEASAIAVIYSLALAMIYREITLKKLNDILLDSVVTSSIVLLLVGCSMGMSWAM
TNADVPELINELITRVSDNKWVILFIINIILLIVGTFMDITPAILIFTPIFLPIAQHLGI
DPIHFGIIMVFNLTIGLCTPPVGTILFVGCSIGKVSIDRAIKPLLPMFLALFVVMAIICY
FPQLSLMLPGLFST