Protein Info for ECOLIN_RS14310 in Escherichia coli Nissle 1917

Annotation: peptidoglycan glycosyltransferase PbpC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02073: penicillin-binding protein 1C
  • amino acids 37 to 770 (734 residues), 915.9 bits, E=1.1e-279
• PF00912: Transgly
  • amino acids 60 to 226 (167 residues), 178.1 bits, E=1.8e-56
• PF00905: Transpeptidase
  • amino acids 330 to 519 (190 residues), 44.9 bits, E=1.4e-15
• PF06832: BiPBP_C
  • amino acids 687 to 767 (81 residues), 65.1 bits, E=6.6e-22

Best Hits

Swiss-Prot: 98% identical to PBPC_ECOLI: Penicillin-binding protein 1C (pbpC) from Escherichia coli (strain K12)

KEGG orthology group: K05367, penicillin-binding protein 1C [EC: 2.4.1.-] (inferred from 98% identity to eco:b2519)

MetaCyc: 98% identical to peptidoglycan glycosyltransferase PbpC (Escherichia coli K-12 substr. MG1655)
Peptidoglycan glycosyltransferase. [EC: 2.4.1.129]

Predicted SEED Role

"Penicillin-insensitive transglycosylase (EC 2.4.2.-) & transpeptidase PBP-1C" in subsystem Peptidoglycan Biosynthesis (EC 2.4.2.-)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
• peptidoglycan maturation (meso-diaminopimelate containing) (6/12 steps found)

KEGG Metabolic Maps

• Anthocyanin biosynthesis
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Carotenoid biosynthesis - General
• Flavone and flavonol biosynthesis
• Flavonoid biosynthesis
• Fructose and mannose metabolism
• Glycerolipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - lacto and neolacto series
• Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
• High-mannose type N-glycan biosynthesis
• Keratan sulfate biosynthesis
• Lipopolysaccharide biosynthesis
• N-Glycan biosynthesis
• O-Glycan biosynthesis
• O-Mannosyl glycan biosynthesis
• Peptidoglycan biosynthesis
• Purine metabolism
• Sphingolipid metabolism
• Zeatin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-, 2.4.1.129, 2.4.2.-

Use Curated BLAST to search for 2.4.1.- or 2.4.1.129 or 2.4.2.-

Protein Sequence (770 amino acids)

>ECOLIN_RS14310 peptidoglycan glycosyltransferase PbpC (Escherichia coli Nissle 1917)
MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARVVVAQDGTPLWRFADAD
GIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTLT
MQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLYLNRAPFGGTLQGIGAASWAY
LGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAARNKVLERMAVQGVWSRERVKES
REEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGRLPPRSS
LAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLALDEGLIHP
ASLLQDVPRRTGDYRPGNFDSGFHGPISMSEALVRSLNLPAVQVLEAYGPKRFAAKLRNV
GLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQPDDPLLERPLMSS
GAAWIIRRIMADEAQPLPDGALPRVAPLAWKTGTSYGYRDAWAIGVNARYVIGIWTGRPD
GTPVVGQFGFASAVPLLNQVNNILLSRSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNC
RRRLATWLLDGSQPPTLLLPEQEGINGIRFPIWLDENGKRVAADCPQARQEMINVWPLPL
EPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDGAIIKRLPGAAAATLPLQSSG
GAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDDVGQIATVKFVMQ