Protein Info for ECOLIN_RS13085 in Escherichia coli Nissle 1917
Annotation: 2-keto-3-deoxy-L-rhamnonate aldolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to RHMA_ECOL6: 2-keto-3-deoxy-L-rhamnonate aldolase (rhmA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K12660, 2-dehydro-3-deoxy-L-rhamnonate aldolase [EC: 4.1.2.-] (inferred from 98% identity to eco:b2245)MetaCyc: 98% identical to 2-keto-3-deoxy-L-rhamnonate aldolase (Escherichia coli K-12 substr. MG1655)
RXN0-5433 [EC: 4.1.2.53]
Predicted SEED Role
No annotation
MetaCyc Pathways
- L-rhamnose degradation II (4/8 steps found)
KEGG Metabolic Maps
- Fructose and mannose metabolism
- Lysine degradation
- Naphthalene and anthracene degradation
- Pentose phosphate pathway
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Tyrosine metabolism
- alpha-Linolenic acid metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.1.2.-
Use Curated BLAST to search for 4.1.2.- or 4.1.2.53
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (267 amino acids)
>ECOLIN_RS13085 2-keto-3-deoxy-L-rhamnonate aldolase (Escherichia coli Nissle 1917) MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLLIDGEHAPNTIQDLYHQ LQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTADQARQVVSATRYPPYGER GVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDNLDEILDVEGIDGVFIGPADL SASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAVAPDMAQQCLAWGANFVAVGVDT MLYSDALDQRLAMFKSGKNGPRVKGSY