Protein Info for ECOLIN_RS06450 in Escherichia coli Nissle 1917

Annotation: N-acetylglucosamine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00480: ROK
  • amino acids 2 to 300 (299 residues), 233.4 bits, E=4.3e-73
• PF02685: Glucokinase
  • amino acids 6 to 256 (251 residues), 30.4 bits, E=2.2e-11

Best Hits

Swiss-Prot: 100% identical to NAGK_ECOL6: N-acetyl-D-glucosamine kinase (nagK) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00884, N-acetylglucosamine kinase [EC: 2.7.1.59] (inferred from 99% identity to eco:b1119)

MetaCyc: 99% identical to N-acetyl-D-glucosamine kinase (Escherichia coli K-12 substr. MG1655)
N-acetylglucosamine kinase. [EC: 2.7.1.59]

Predicted SEED Role

"N-acetyl-D-glucosamine kinase (EC 2.7.1.59)" (EC 2.7.1.59)

MetaCyc Pathways

• N-acetylglucosamine degradation II (3/3 steps found)
• chitin derivatives degradation (4/8 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.59

Protein Sequence (303 amino acids)

>ECOLIN_RS06450 N-acetylglucosamine kinase (Escherichia coli Nissle 1917)
MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVCELVAEADRRFGCKGSV
GIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRLDNDANCFALSEAWDDEFT
QYPLVMGLILGTGVGGGLIFNGRPITGKSYITGEFGHMRLPVDALTMMGLDFPLRRCGCG
QYGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQGDEQARAHVERYLDLLAVCLGNI
LTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPVARVPRIERARHGDAGGMRGAAFLH
LTD