Protein Info for Dsui_3498 in Dechlorosoma suillum PS

Annotation: isoleucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 930 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 848 (834 residues), 893.4 bits, see alignment E=8.3e-273 PF00133: tRNA-synt_1" amino acids 27 to 649 (623 residues), 466.4 bits, see alignment E=2.4e-143 PF09334: tRNA-synt_1g" amino acids 58 to 198 (141 residues), 41.7 bits, see alignment E=2e-14 PF08264: Anticodon_1" amino acids 693 to 847 (155 residues), 99.1 bits, see alignment E=6.6e-32 PF06827: zf-FPG_IleRS" amino acids 891 to 918 (28 residues), 39.1 bits, see alignment (E = 1.6e-13)

Best Hits

Swiss-Prot: 75% identical to SYI_AZOSB: Isoleucine--tRNA ligase (ileS) from Azoarcus sp. (strain BH72)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 75% identity to azo:azo1205)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QLB3 at UniProt or InterPro

Protein Sequence (930 amino acids)

>Dsui_3498 isoleucyl-tRNA synthetase (Dechlorosoma suillum PS)
MADYKNSLNLPDTPFPMRGDLPKREPQWVQQWQEKKLYQKIREICAGRPKFILHDGPPYA
NGNLHLGHALNKILKDIIVRSKTLAGFDAPYVPGWDCHGLPIEHKIEVTHGKNLPADKVR
ELCRSYAAEQVEVQKKEFIRLGVLGDWDNPYLTMNFANEAGEIRALAEMVKQGYVFKGLK
PVNWCFDCGSALAEAEVEYADKKSPTIDVAFPVSEADAPKLAAAFGLNALPKPAYAVIWT
TTPWTIPANQALNIHPEHEYALVDAGDRLLVLAKELVADCLKRYGCEGTVLATVKGKALE
RLEFRHPFYDRVSVVYLADYVGLDAGTGIVHCAPAHGVDDFNAWRHYGRGNDEIISIVKG
DGVYVDELPFFGGLSIWKANQAICDKLSEVGCLLSHGNVSHSYMHCWRHKTPVIYRATAQ
WFVGMDRLPTDGKSLREKALAGVEATAFYPAWGKPRLQSMIANRPDWCISRQRNWGVPIP
FFLHKETGELHPRTVELMEEVAQRVEKEGIEAWFKLDAAELLGAEAAQYEKISDTLDVWF
DSGTTHWHVLRGSHNDGHATGPRADLYLEGSDQHRGWFHSSLLTGAAIDGHPPYKALLTH
GFTVDQQGRKMSKSLGNTILPQEVSDKMGADILRLWVAATDYSGELAMSKEILARVVEAY
RRIRNTLRFLLANIADFDAAKDMLPVAEWFEIDRYALALTRQLQTQAQADYDRYEFHRVV
QALQTFCSEDLGGFWLDILKDRLYTTAPASKARRSAQSALWHVTQSFIRLMAPILSFTAE
EVWQILSGDADQSIMFHTWHDLPAQAGEGELLAKWTLIREARGEVTKALEALREEGKIGS
SLQAEVRISAEGDKYAALASLGDDLRFVLICSKVSVAQGADAIAAEASTQKKCERCWHYR
EDVGSNPEHPELCGRCDSNLHGEGEVRERA