Protein Info for Dsui_3481 in Dechlorosoma suillum PS

Annotation: aspartate ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00839: aspartate ammonia-lyase
  • amino acids 6 to 465 (460 residues), 729.1 bits, E=1.2e-223
• PF00206: Lyase_1
  • amino acids 14 to 344 (331 residues), 330.7 bits, E=1e-102
• PF10415: FumaraseC_C
  • amino acids 410 to 462 (53 residues), 71.6 bits, 5.9e-24

Best Hits

Swiss-Prot: 59% identical to ASPA_HELPJ: Aspartate ammonia-lyase (aspA) from Helicobacter pylori (strain J99 / ATCC 700824)

KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 86% identity to azl:AZL_b05180)

MetaCyc: 57% identical to aspartate ammonia-lyase (Escherichia coli K-12 substr. MG1655)
Aspartate ammonia-lyase. [EC: 4.3.1.1]

Predicted SEED Role

"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)

MetaCyc Pathways

• L-glutamate degradation II (2/2 steps found)
• L-aspartate degradation II (aerobic) (2/3 steps found)
• L-aspartate degradation III (anaerobic) (2/3 steps found)
• superpathway of L-aspartate and L-asparagine biosynthesis (2/4 steps found)
• L-canavanine degradation II (1/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.1

Sequence Analysis Tools

See G8QL96 at UniProt or InterPro

Protein Sequence (472 amino acids)

>Dsui_3481 aspartate ammonia-lyase (Dechlorosoma suillum PS)
MNQPHRVEHDLLGDRKVPAEAYYGVHTLRAVENFPITGTPISIYPDLIRALAQIKLAAAK
ANEQLGLLEAPLAQAIVAACQEVIAGKLHEQFVVDVIQGGAGTSTNMNANEVIANRALEI
MGHAKGEYQHLHPNQHVNMSQSTNDVYPSALKLATYVGIFRLVDALAYLRRTFERKAEEF
KDVLKMGRTQLQDAVPMTLGQEFSTYAVMLGEDEERLKEAALLIREINLGATAIGTGINA
HPDYAPLVCRLLAETSGVPVITAPNLVEATQDCGSFVQLSGVLKRVAVKLSKTCNDLRLL
SSGPRAGFGEINLPPRQAGSSIMPGKVNPVIPEVVNQIAFEVIGNDQTVTFAAEAGQLQL
NAFEPIIAHSLFKSVNHLRQGCLTLADRCVDGITANRDALRARVENSIGIVTALNPYIGY
AAATSVAAEALESGKGVAEIVLARGLMSRAELDDVLRPEVLTQPRALPHRQG