Protein Info for Dsui_3392 in Dechlorosoma suillum PS

Annotation: carboxylate-amine ligase, YbdK family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 transmembrane" amino acids 247 to 264 (18 residues), see Phobius details TIGR02050: carboxylate-amine ligase, YbdK family" amino acids 11 to 298 (288 residues), 302.8 bits, see alignment E=1.3e-94 PF04107: GCS2" amino acids 12 to 270 (259 residues), 189.8 bits, see alignment E=3.6e-60

Best Hits

Swiss-Prot: 51% identical to GCS2_BURTA: Putative glutamate--cysteine ligase 2 (BTH_I0001) from Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)

KEGG orthology group: K06048, carboxylate-amine ligase [EC: 6.3.-.-] (inferred from 52% identity to bgd:bgla_1g00550)

Predicted SEED Role

"FIG074102: hypothetical protein"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QKI3 at UniProt or InterPro

Protein Sequence (374 amino acids)

>Dsui_3392 carboxylate-amine ligase, YbdK family (Dechlorosoma suillum PS)
MLEFKSVSPYTLGVELELQVINPHTQDLTRGGPDLLRALEKVAHPGEIKPEITESMIELN
SSPHARHDSLLEELHAMRDAVVHTAGSLNLAIAGGGTHPFHRWHERRIFPTERFQQLSSL
YGYLAKQFTVFGQHIHVACESGDDAVYLTHQLSRYIPHFIALSAASPFSQGQDTSFETSR
LHAVSAFPLSGHMPPVSTWAGFEEFFAKMLGYGIIGSIKDFYWDIRPKPEFGTVEVRICD
TPLEVDTAAALAAFVQALAFWLLVERPYPADRVEEGLYLVYGYNRFQATRFGFDATLVDP
RSQQSLVLRDDLLATLERLQPFADILGSRTPLARLAAMAGSGANGSAWLRARHEESGALE
DVVRHSARCWAGGV