Protein Info for Dsui_3294 in Dechlorosoma suillum PS

Annotation: isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 transmembrane" amino acids 348 to 370 (23 residues), see Phobius details PF20423: AceK_regulatory" amino acids 44 to 344 (301 residues), 401.4 bits, see alignment E=2.5e-124 PF06315: AceK_kinase" amino acids 345 to 600 (256 residues), 409.6 bits, see alignment E=5.6e-127

Best Hits

Swiss-Prot: 75% identical to ACEK_AZOSB: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Azoarcus sp. (strain BH72)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 75% identity to azo:azo1145)

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QJF6 at UniProt or InterPro

Protein Sequence (615 amino acids)

>Dsui_3294 isocitrate dehydrogenase kinase/phosphatase (Dechlorosoma suillum PS)
MEPVRAPFPREAGLAGAKISAFFRVPPVPMDISTAATANRIAIALIDGFNKHYRLFRQTS
REAKFRFESGDWLAVHKAVKDRIRFYDDRVEECVERLRDEFRAEALDDATWQQVKLLYIG
LLLNHKQPELAETFFNSVCCKILHRTYFHNDFIFVRPAISTENIESNPPTYSSYYPGEGG
LAETMRQIILDFDWQRDFEDLERDVDYLYRTIRTHLGGELPAREANCQVQVLYSAFYRNK
GAYIIGKAINGNQEYPFAIPVLHTPGGKLYLDTILLDAWRISILFSLSRAYFMVDMEVPS
GYVQFLRSIMPGKPRSELYTMLGLGKQGKTMFFRDLIYHLRHSEDKFIVAPGIRGLVMLV
FTLPSFPYVFKIIKDVFGSSKEVDHATVKYKYQLVKQVDRVGRMADTLEFSYAALPRSRF
DPALLEELRQQVPSLLEEDGEDLIIKHLYIERRMTPLNIYLDNAPEEKVEAAVKDYGNAI
RELAIANIFPGDMLWKNFGVTRYNRIVFYDYDEIEHMTDMNFRAIPEAPYPELEMSGEPW
YSVARNDVFPEEFATFLLGSRPVRQAFLKHHRDLLQPEFWQAAQEQIRQGRVEDFFPYPE
ELRFSRLYAAGGENA