Protein Info for Dsui_3282 in Dechlorosoma suillum PS

Annotation: formate dehydrogenase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1017 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 2 to 1016 (1015 residues), 1554.1 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 44 to 103 (60 residues), 58.5 bits, see alignment 7.8e-20 PF00384: Molybdopterin" amino acids 107 to 589 (483 residues), 103.5 bits, see alignment E=1.9e-33 PF01568: Molydop_binding" amino acids 895 to 1010 (116 residues), 52.2 bits, see alignment E=8.8e-18

Best Hits

Swiss-Prot: 67% identical to FDOG_ECOLI: Formate dehydrogenase-O major subunit (fdoG) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 67% identity to avn:Avin_03810)

MetaCyc: 67% identical to formate dehydrogenase O subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QJE4 at UniProt or InterPro

Protein Sequence (1017 amino acids)

>Dsui_3282 formate dehydrogenase, alpha subunit (Dechlorosoma suillum PS)
MQVTRRQFFKVSAAGLSASSLVALGFSPATALAEVRQYKLTRAKEIRNTCTYCSVGCGLL
MYSLGDGAKNAKQEIIHIEGDPDHPVSRGSLCPKGAGLLDFIHSPNRLKYPEVREAGSKE
WKRISWHEAVERIAKHMKADRDANFVAKNADGVTVNRWLSTAMLTASASSNETGILTQKF
MRSLGIVGTDAQARVCHGPTVAALAATFGRGAMTNTWVDIKNADFILIMGGNPAEAHPVG
FKWAIEAKKKGAKLIVVDPRFNRSAAVADEFVPIRAGSDIAFLGGIINWLVSNDKIQWDY
VKSFTNASFIVNEGFTFDEGLFSGYDEAKGKYDRGSWSYELDAKGQAKTDPTLQHPRCVW
NLMKAHYARYTPEVVSDLTGSPKEGFLSVCKHLGETCVPTKVGTILYALGWTQHTVGAQN
IRTMAMIQLLLGNIGMPGGGVNALRGHSNIQGLSDLGLLSTSLPGYLTLPSEKAHPTFAD
YLEKTTPKALQPGQLNYWGNTPKFFVSLMKWFWGDKATKENNWGYDWLPKWDKMYDVLQV
MDLMHQGKINGFIVQGFNPLTSFPDAKKTAADFAKLKYMVIIDPIATETSTFWQNQGELN
DVDPASIQTEVFRLPSTCFAEEDGAIVNSSRWLQWHWKGADAPGEAKTDQEIIAELFMAL
KKLYQEQGGAVPEPILNLSWPYKNPYDPTPEELAKELNGRALADIPDPKNPGQFLVKKGE
QIPGFAVLQDDGSTMSACWIFAGCWTQAGNQMARRDNTDTGLGNTPGWAWAWPANRRIIY
NRASCDAAGKPWDPKRKLIAWNGEKWAGADVPDFKVDAAPDSGMNPFIMNPEGVGRLFAT
DKLVDGPFPEHYEPMESPLGTNPLHPKVVTSPAVRIFKADKERLGTHKEFPYVGTTYRLT
EHFQFWTKSVRLSAIAQPEQFVEVGEALAAEKGIKAGDWVKVSSKRGYIKAKAVVTKRIK
ALTVNKQTVHQVGIPLHWGWETVAKKGFLSNTLPPAVGDCNTQTPEYKAFLVNIEKA