Protein Info for Dsui_3248 in Dechlorosoma suillum PS
Annotation: argininosuccinate synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 91% identical to ASSY_AZOSB: Argininosuccinate synthase (argG) from Azoarcus sp. (strain BH72)
KEGG orthology group: K01940, argininosuccinate synthase [EC: 6.3.4.5] (inferred from 91% identity to tmz:Tmz1t_2674)MetaCyc: 49% identical to Argininosuccinate synthase (Homo sapiens)
Argininosuccinate synthase. [EC: 6.3.4.5]
Predicted SEED Role
"Argininosuccinate synthase (EC 6.3.4.5)" in subsystem Arginine Biosynthesis extended (EC 6.3.4.5)
MetaCyc Pathways
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
- superpathway of arginine and polyamine biosynthesis (13/17 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
- urea cycle (4/5 steps found)
- nitric oxide biosynthesis II (mammals) (2/3 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- canavanine biosynthesis (2/4 steps found)
- L-arginine biosynthesis IV (archaea) (4/9 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Arginine and proline metabolism
- Urea cycle and metabolism of amino groups
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.4.5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8QJB1 at UniProt or InterPro
Protein Sequence (409 amino acids)
>Dsui_3248 argininosuccinate synthase (Dechlorosoma suillum PS) MSDIKKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADLGQGEELEPARTKALKFGIKPE NIFIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIAKRLIEITNLTGADAISHG ATGKGNDQVRFELGAYALKPGVKVIAPWREWDLLSREKLLAYAEQHGIPVEMKHKQGGSP YSMDANLLHISYEGRHLENPAAEAEEDMWRWTVSPENAPDAAEYLDLEFVQGDLVAINGQ NMKAHELLAKLNELGGKHGIGRLDLVENRYVGMKSRGCYETPGGTILLRAHRAIESITLD REVAHLKDDLMPRYASLVYNGYWWAPERKALQTLIDHTQQTVNGTVRLKLYKGNVIVVGR DSKTDSLFDPTIATFEDDAGAYDQKDAGGFIKLNALRMRIAANLKARKG