Protein Info for Dsui_3189 in Dechlorosoma suillum PS

Annotation: folylpolyglutamate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 29 to 423 (395 residues), 339.9 bits, E=1.3e-105
• PF08245: Mur_ligase_M
  • amino acids 50 to 269 (220 residues), 35.7 bits, E=9.2e-13
• PF02875: Mur_ligase_C
  • amino acids 296 to 416 (121 residues), 83 bits, E=3.4e-27

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 73% identity to dar:Daro_0873)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of chorismate metabolism (48/59 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

See G8QIR1 at UniProt or InterPro

Protein Sequence (438 amino acids)

>Dsui_3189 folylpolyglutamate synthase/dihydrofolate synthase (Dechlorosoma suillum PS)
MSSPTTLADWLTHLESLHPKGQAGIELGLERVARVKAALGQTEFCPVVTVGGTNGKGSVC
AYLENILDRAGCKVGSYSSPHLLRYNERVRLNGQPVADEALCAAFARVEAARGDEALTYF
EFGTLAAWEVFAAAGVEAIVLEVGLGGRLDAVNIYEPDVAVVTGIALDHTDWLGPTREDI
GREKAGIFRPGKPAICADAKPPQSLLDHAAAIGAELKLIGRNFGFEKSAEDRLQWRYWLR
QGEALQRRALAFPGLRGTAQLANASAALTALDYLKDRLPVSMGAIRAGLIETELPGRFQV
LPGQPAVVLDVAHNPQSAQVLADNLGNMGFYNRTHAVVGMLGDKDIATTLAALKGKVDTW
LLADLAVPRGASAVQLAEAVAGLGGEVHCHDSPYAAWQAALKQAAENDRILVFGSFHTVA
GVLPHVGQRHRQPGQTPS