Protein Info for Dsui_3084 in Dechlorosoma suillum PS

Annotation: cysteinyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 TIGR00435: cysteine--tRNA ligase" amino acids 2 to 475 (474 residues), 568.8 bits, see alignment E=5.4e-175 PF01406: tRNA-synt_1e" amino acids 15 to 313 (299 residues), 453.8 bits, see alignment E=7.7e-140 PF09334: tRNA-synt_1g" amino acids 36 to 118 (83 residues), 20.9 bits, see alignment E=3.5e-08 amino acids 244 to 297 (54 residues), 27.9 bits, see alignment 2.6e-10 PF00133: tRNA-synt_1" amino acids 256 to 303 (48 residues), 23.9 bits, see alignment 4.2e-09 PF09190: DALR_2" amino acids 342 to 397 (56 residues), 71.9 bits, see alignment 1.3e-23

Best Hits

Swiss-Prot: 78% identical to SYC_RALSO: Cysteine--tRNA ligase (cysS) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 79% identity to hse:Hsero_1870)

MetaCyc: 60% identical to cysteine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QHM5 at UniProt or InterPro

Protein Sequence (476 amino acids)

>Dsui_3084 cysteinyl-tRNA synthetase (Dechlorosoma suillum PS)
MLKIYNTIAREKQEFVPIEAGKVKMYVCGMTVYDFCHLGHARVLVVFDAVYRWLRASGLD
VTYVRNITDIDDKIIRRAVENGETIGQLTDRFIAAMDEDAAALGVQKPTHEPRATEYVPQ
MLGLIGQLEANGLAYKAADGDVNFSVRKFPGYGRLSGKSLDDLRAGERVEVDSAKQDPLD
FVLWKHAKAGEPAWESPWGAGRPGWHIECSAMSSDLLGKHFDIHGGGQDLQFPHHENEIA
QSEGAHGCTFVNYWMHNGFVRVDDEKMSKSLGNFFTIRDVLKKYDAEVVRFFILRAHYRS
PLNYSDAHLDDARQALTRLYTALKAAPAEAVAVDWNEAHAQRFQAAMDDDFNTPEALAVL
FDLANEANRSKDSAVAAQLKALAGVLGLLARSPEDYLKGRVVYGSGNATMSLSGNGEGTL
LVGGGISESDIEARIQARIDAKKAKNFAEADRLRAELLAAGIVLEDSAQGTTWRRA