Protein Info for Dsui_3064 in Dechlorosoma suillum PS

Annotation: carbamoyl-phosphate synthase, large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1045 (1044 residues), 1562.6 bits, E=0
• PF25596: CPSase_L_D1
  • amino acids 7 to 125 (119 residues), 162.5 bits, E=1.7e-51
  • amino acids 552 to 664 (113 residues), 166.4 bits, E=1.1e-52
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 246.5 bits, E=1e-76
  • amino acids 668 to 869 (202 residues), 130.4 bits, E=3.5e-41
• PF02787: CPSase_L_D3
  • amino acids 422 to 500 (79 residues), 89.2 bits, 7.5e-29
• PF02655: ATP-grasp_3
  • amino acids 667 to 840 (174 residues), 28.4 bits, E=8e-10
• PF02222: ATP-grasp
  • amino acids 675 to 840 (166 residues), 39.5 bits, E=2.4e-13
• PF07478: Dala_Dala_lig_C
  • amino acids 693 to 839 (147 residues), 34.6 bits, E=7.3e-12
• PF15632: ATPgrasp_Ter
  • amino acids 798 to 867 (70 residues), 29.2 bits, 3.6e-10
• PF02142: MGS
  • amino acids 951 to 1037 (87 residues), 78.6 bits, E=1.6e-25

Best Hits

Swiss-Prot: 60% identical to CARB_ARCFU: Carbamoyl-phosphate synthase large chain (carB) from Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

MetaCyc: 70% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of arginine and polyamine biosynthesis (13/17 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See G8QHK5 at UniProt or InterPro

Protein Sequence (1068 amino acids)

>Dsui_3064 carbamoyl-phosphate synthase, large subunit (Dechlorosoma suillum PS)
MPKRTDIKSILIIGAGPIIIGQACEFDYSGAQACKALKAEGYRVILVNSNPATIMTDPET
ADVTYIEPISWKVVEKIIEKERPDALLPTMGGQTALNCALDLAKHGVLEKFGVELIGASE
EAIDKAEDREKFKAAMTKIGLGSARSAVAHSMEEALQVQAMIGFPAIIRPSFTLGGSGGG
IAYNKEEFVTICERGLEASPTKELLIEESLIGWKEYEMEVVRDSKDNCIIICSIENLDPM
GVHTGDSITVAPAQTLTDKEYQIMRNASIAVLREIGVDTGGSNVQFAISPKDGRMIVIEM
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELANEITGGKTPASFEPSIDYVVTKVP
RFAFEKFPTADFHLTTQMKSVGEVMAIGRTLQESLQKALRGLEVGVDGFDEKTTDREVIE
TELAEPGPERIWYVGDAFRIGMTLDEIHRLTHIDPWFLAQIEDLHLKAKSLAGRSVDSLS
REELLVLKKCGFSDKRLAKLLATTQTAVRERRHALNVRPVFKRVDTCAAEFATNTAYMYS
TYEDECEAQPSDKKKIMVLGGGPNRIGQGIEFDYCCVHAAMAMREDGYETIMVNCNPETV
STDYDTSDRLYFEPLTLEDVLEVVNVEKPVGVIVQYGGQTPLKLARDLEANGVPIIGTSP
DMIDAAEDRERFQKLLHELGLKQPPNRTARNEADALALAQEIGYPLVVRPSYVLGGRAME
IVHQQSDLERYMREAVKVSNDSPVLLDRFLNDACEVDVDALSDGDEVIIGGVMEHIEQAG
VHSGDSACSLPPYSLTKEVTDELRRQTKLMAKALNVCGLMNVQFAIQNDTVYVLEVNPRA
SRTVPFVSKATGLQLAKIAARCMAGQSLKSQGITKEVIPPYFSVKEAVFPFVKFPGVDTI
LGPEMKSTGEVMGVGTTFAEAFVKSQLAAGVKLPTGGKVFLSVKDSDKTKAVDVARDLHA
AGFTILATRGTGAAMEAAGIPVTVVNKVTEGRPHIVDMIKNNEIALIINTVDEKRQAIND
SRSIRTSGLAARVTMYTTIWGAEAAAAGIRQGGELVVYPIQALHAQIS