Protein Info for Dsui_2386 in Dechlorosoma suillum PS

Annotation: pyruvate-formate lyase-activating enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 TIGR04337: AmmeMemoRadiSam system radical SAM enzyme" amino acids 17 to 364 (348 residues), 618.5 bits, see alignment E=1.4e-190 PF04055: Radical_SAM" amino acids 91 to 245 (155 residues), 61.4 bits, see alignment E=6.4e-21

Best Hits

KEGG orthology group: K04069, pyruvate formate lyase activating enzyme [EC: 1.97.1.4] (inferred from 74% identity to dar:Daro_1969)

Predicted SEED Role

"COG1180: Radical SAM, Pyruvate-formate lyase-activating enzyme like"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.97.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QLV6 at UniProt or InterPro

Protein Sequence (366 amino acids)

>Dsui_2386 pyruvate-formate lyase-activating enzyme (Dechlorosoma suillum PS)
MNPDHTPAVAESAHPGRWWHALEDGRLQCDLCPRDCRLHEGQRGACFVRQARDGAMILTT
YGRSSGFCLDPVEKKPLNHFHPGTPILSFGTAGCNLACKFCQNWDISKSRDMDRLMDAAS
PAAIAAAARRLEAPAVAFTYNDPVIFAEYAMDTALACREAGIRSVAVTAGYIHAEPRKEF
FALMDAANVDLKAFTEDFYVKLCGGHLQPVLDTLAHIHHLTDCWLEITTLLIPGHNDSDR
EIAQLAGWIERELGPEVPLHFSAYHPDWKFLEAPETPPATLSRARRIARDAGLHFVYTGN
VHDTAGGTTDCPRCHAPLIVRDWYTLKQYRLTASGACPDCGHQLAGRFGPGPGDFGARRQ
PVRLGT