Protein Info for Dsui_2312 in Dechlorosoma suillum PS

Annotation: 3-hydroxybutyrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00106: adh_short" amino acids 5 to 198 (194 residues), 204.1 bits, see alignment E=3.1e-64 TIGR01963: 3-hydroxybutyrate dehydrogenase" amino acids 5 to 259 (255 residues), 358.6 bits, see alignment E=8.1e-112 PF01370: Epimerase" amino acids 7 to 239 (233 residues), 24.4 bits, see alignment E=3.6e-09 PF08659: KR" amino acids 8 to 165 (158 residues), 54.7 bits, see alignment E=2.5e-18 PF13561: adh_short_C2" amino acids 13 to 255 (243 residues), 181.5 bits, see alignment E=4.1e-57

Best Hits

Swiss-Prot: 51% identical to BDHA_CUPNH: D-beta-hydroxybutyrate dehydrogenase (hbdH1) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00019, 3-hydroxybutyrate dehydrogenase [EC: 1.1.1.30] (inferred from 62% identity to aex:Astex_1971)

Predicted SEED Role

"D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30)" in subsystem Polyhydroxybutyrate metabolism (EC 1.1.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QL16 at UniProt or InterPro

Protein Sequence (260 amino acids)

>Dsui_2312 3-hydroxybutyrate dehydrogenase (Dechlorosoma suillum PS)
MLSGKIALVTGSTSGIGLAVARALARNGAAVMLNGSRPAAEAEGLRAAMAAEFGVKVAYT
SADLADAASVRALAAAAEKQLGVVDILVNNAGIQHVAAVDEFPEEKWDQLIAINLSSVFH
ATKAVLPGMKARNWGRIVNIASAHGLVASPFKAPYTASKHAVVGFSKAVALEVAETGITC
NAVCPGYVRTPLVEKQVAAQAKVHNLPEDQVIRDVILAAQPNKRFLEADDLAEFVLFLCS
PAGAGMTGAALPMDGAWTAR