Protein Info for Dsui_2293 in Dechlorosoma suillum PS

Annotation: poly(3-hydroxyalkanoate) synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF12551: PHBC_N" amino acids 29 to 70 (42 residues), 60.9 bits, see alignment 1.2e-20 PF07167: PhaC_N" amino acids 106 to 274 (169 residues), 189.5 bits, see alignment E=6.3e-60 PF00561: Abhydrolase_1" amino acids 248 to 515 (268 residues), 54.5 bits, see alignment E=2.1e-18

Best Hits

KEGG orthology group: K03821, polyhydroxyalkanoate synthase [EC: 2.3.1.-] (inferred from 66% identity to tmz:Tmz1t_1149)

Predicted SEED Role

"Polyhydroxyalkanoic acid synthase" in subsystem Polyhydroxybutyrate metabolism

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QKZ7 at UniProt or InterPro

Protein Sequence (594 amino acids)

>Dsui_2293 poly(3-hydroxyalkanoate) synthetase (Dechlorosoma suillum PS)
MPPAVATSSLLPLISPAAQVLTPLQADSATQRLDQHVHATIAQLTNSLSPLTMAMAMIDW
AGHLAVSPGKQLELLKLAATEAAALQAAQLSMLNEGPDFVAPWSTDRRFKEEAWHRWPFN
VMQQSFLLTERWWKAATHGIWGVSAHHERIVSFAARQILDLLSPGNSPLTNPVVLEHTIR
EGGQNLWRGMAHLQEDLQRAMNNEPPAGSEDYVVGRDVAITPGKVVARTRLMELIQYSPT
TAKVHPEPILIVPAWIMKYYILDLSPHNSLIKFLVDQGHTVFCISWRNPDEEDRDQGMDD
YLENGFFAALQAINDIVPKRRVHATGYCLGGTLLAIAAAAMARDGDERLASITLLAAQTD
FTEPGELALFIDDSQISLLEAQMAEKGYLSAGQMAGAFQLLRSYDLLWSRMVSQYLLGER
GAMNDLMAWNADTTRMPARMHSQYLRQLFLNDDLSEGRYQVGGRPVSLGDITIPVFAVGT
ESDHVAPWRSVHKIHYLSPAEVTFVLTSGGHNAGIVSEPGHPRRHYRLHTRPAGEVYVAP
EQWLEETPTREGSWWTAWQEWLAARSAAPAAPAKTGSKRYPPLEDAPGSYVLLK