Protein Info for Dsui_2218 in Dechlorosoma suillum PS

Annotation: 2-oxoglutarate dehydrogenase complex dihydrolipoamide succinyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01347: dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex
  • amino acids 3 to 401 (399 residues), 556.5 bits, E=2.1e-171
• PF00364: Biotin_lipoyl
  • amino acids 4 to 76 (73 residues), 66.6 bits, E=2.2e-22
• PF02817: E3_binding
  • amino acids 115 to 146 (32 residues), 47.3 bits, see alignment (E = 2.8e-16)
• PF00198: 2-oxoacid_dh
  • amino acids 172 to 400 (229 residues), 275.2 bits, E=6.3e-86

Best Hits

Swiss-Prot: 74% identical to ODO2_CUPNH: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (sucB) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K00658, 2-oxoglutarate dehydrogenase E2 component (dihydrolipoamide succinyltransferase) [EC: 2.3.1.61] (inferred from 86% identity to azo:azo1556)

Predicted SEED Role

"Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61)" in subsystem TCA Cycle (EC 2.3.1.61)

MetaCyc Pathways

• 2-oxoglutarate decarboxylation to succinyl-CoA (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Lysine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.61

Sequence Analysis Tools

See G8QK98 at UniProt or InterPro

Protein Sequence (402 amino acids)

>Dsui_2218 2-oxoglutarate dehydrogenase complex dihydrolipoamide succinyltransferase (Dechlorosoma suillum PS)
MIIDVKVPQLSESVAEATLVSWHKKAGEAVSRDENLIDIETDKVVLELPAPDAGVIVKII
KGDGDTVVSGEVIAQIDTEAKAAAGAAPAAAAAPAAAAPAPVAAAAAPAAAAGTASPAAR
KILDEKGIAAADVSGTGRGGRVTKEDAVGAQAKAAAPAVAAPAVSIPLGDRPEQRVPMTR
LRARIAERLLQSKNENAILTTFNEVNMAPVMALRKQYGDKFEKEHGVRLGFMGFFVKAAV
AALKKYPILNASVDGNDIVYHGYIDIGIAVGSPRGLVVPILRDADQMSIADIEKKIAEYG
QKAKDGKISIEELTGGTFSISNGGVFGSMLSTPIINPPQSAILGIHATKDRAVVENGQVV
VRPINYLAMSYDHRIIDGREAVLGLVTMKEALEDPARLLLGV