Protein Info for Dsui_2197 in Dechlorosoma suillum PS

Annotation: endopeptidase Clp ATP-binding regulatory subunit ClpX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 424 TIGR00382: ATP-dependent Clp protease, ATP-binding subunit ClpX" amino acids 10 to 413 (404 residues), 671.7 bits, see alignment E=2e-206 PF06689: zf-C4_ClpX" amino acids 14 to 49 (36 residues), 70.4 bits, see alignment 4.1e-23 PF00493: MCM" amino acids 70 to 195 (126 residues), 23.3 bits, see alignment E=1.4e-08 PF07724: AAA_2" amino acids 114 to 312 (199 residues), 119.3 bits, see alignment E=8.4e-38 PF07728: AAA_5" amino acids 116 to 193 (78 residues), 28.2 bits, see alignment E=8e-10 PF00004: AAA" amino acids 117 to 234 (118 residues), 61.3 bits, see alignment E=6.1e-20 PF10431: ClpB_D2-small" amino acids 318 to 389 (72 residues), 36.7 bits, see alignment E=1.5e-12

Best Hits

Swiss-Prot: 84% identical to CLPX_DECAR: ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K03544, ATP-dependent Clp protease ATP-binding subunit ClpX (inferred from 85% identity to app:CAP2UW1_2271)

MetaCyc: 76% identical to ATP-dependent Clp protease ATP-binding subunit ClpX (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpX" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QJT8 at UniProt or InterPro

Protein Sequence (424 amino acids)

>Dsui_2197 endopeptidase Clp ATP-binding regulatory subunit ClpX (Dechlorosoma suillum PS)
MTEKKSGSAEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIELCNDIIRDEIAGDVGGKG
GKDDLPTPKEICSILDQYVIGQEVAKKILSVAVYNHYKRLRHQGKSADDVELAKSNILLI
GPTGSGKTLLAQTLARLLNVPFVMADATTLTEAGYVGEDVENIIQKLLQKCDYEVEKAQQ
GIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVASVPPQGGRKHPNQDFVQV
DTANILFICGGAFDGLEKVIRNRSTRGGIGFGAEVKSKEDKKAVGEVLRTVEPEDLIKFG
LIPEFVGRLPVIATLDELSEAALVEILIEPKNALIKQYQKLFAMEGAELEVRPAALTAIA
RRALERKTGARGLRSILEGVLLETMYELPGMDNVSKVVIDEGMISGEAKPILIYAEQPKV
SGSN