Protein Info for Dsui_1963 in Dechlorosoma suillum PS

Annotation: phenylalanyl-tRNA synthetase, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 790 transmembrane" amino acids 539 to 557 (19 residues), see Phobius details TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 789 (789 residues), 823.1 bits, see alignment E=1.3e-251 PF01588: tRNA_bind" amino acids 45 to 144 (100 residues), 79.1 bits, see alignment E=5.5e-26 PF03483: B3_4" amino acids 211 to 384 (174 residues), 243.5 bits, see alignment E=2.5e-76 PF03484: B5" amino acids 403 to 468 (66 residues), 74 bits, see alignment E=2.2e-24 PF17759: tRNA_synthFbeta" amino acids 471 to 681 (211 residues), 215.5 bits, see alignment E=1.6e-67 PF03147: FDX-ACB" amino acids 696 to 789 (94 residues), 100.6 bits, see alignment E=1.4e-32

Best Hits

Swiss-Prot: 73% identical to SYFB_DECAR: Phenylalanine--tRNA ligase beta subunit (pheT) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 73% identity to dar:Daro_2672)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QHY2 at UniProt or InterPro

Protein Sequence (790 amino acids)

>Dsui_1963 phenylalanyl-tRNA synthetase, beta subunit (Dechlorosoma suillum PS)
MKFSESWLRTFVNPDLSTDALAHLLTMAGLEVEEQDPVAPAFTQVVVAHVLEVVKHPDAD
RLNVCQVDAGNGPVQIVCGAPNVAAGLKVPCALPGAVLPGDFKIKVAKVRGVESSGMLCS
ARELGIAEDASGLLVLPADAPVGESIRRYLDLDDTLFTLKLTPNRADCLSLTGVAREVSA
LTGVAAVYPVVEPVAPACDARREVVLDAPEACPRYAGRVVRGINAKAPTPEWMQKRLERS
GIRSISAVVDVTNYVMLELGQPLHAFDDAKLQGAIHARLPKAGEKLLLLNEQTIDIDADV
LVIADDAKPLALAGIMGGEESGVTMETTDLFLESAFFTPFAIAGKARRYGFSSDASHRFE
RGVDFAGAVRALERATRLILDICGGQAGPVTEAVAALPQRQPVRLRTARVNKVLGVPFSA
EAIASLLGRLGLPFVQEGGDFVVTPPSFRFDIEIEEDLIEEVVRVHGYDEIPAPPPVARA
AMLPMPEGKRQIQRLRQILADAGYQEVVNFAFVEEGWEKDFGGNADPIRLANPIASQMGV
MRSTLIGGLVATLANNLKRKQSRVRIFETGRSFHKDAAAQPVAGYRQPWKLAGLAAGDAL
PEQWGSAARKVDFFDVKGDLEALLQPFAARFEKAAHPALHPGRAARLLLDGKEIGVVGEL
HPEWVQKYDLPSPPVVFELDLEPLLAGRLPQYAELSRFPAVVRDLAVVVDKGMALQSLLD
GLKAQRPPLVQDIQLFDVYTGKGVDEGKKSLAFRIVMQDTQKTLQDAEVDVALQQLVALL
EHQFAAKLRV