Protein Info for Dsui_1446 in Dechlorosoma suillum PS

Annotation: succinyl-CoA synthetase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01019: succinate-CoA ligase, alpha subunit
  • amino acids 3 to 297 (295 residues), 411.9 bits, E=7.5e-128
• PF02629: CoA_binding
  • amino acids 6 to 102 (97 residues), 82.5 bits, E=4.6e-27
• PF13607: Succ_CoA_lig
  • amino acids 154 to 293 (140 residues), 41.4 bits, E=1.8e-14
• PF00549: Ligase_CoA
  • amino acids 160 to 271 (112 residues), 75.4 bits, E=6.9e-25

Best Hits

Swiss-Prot: 63% identical to SUCD_BACSU: Succinate--CoA ligase [ADP-forming] subunit alpha (sucD) from Bacillus subtilis (strain 168)

KEGG orthology group: K01902, succinyl-CoA synthetase alpha subunit [EC: 6.2.1.5] (inferred from 94% identity to ajs:Ajs_4046)

MetaCyc: 81% identical to 3-sulfinopropionyl-CoA synthetase alpha subunit (Advenella mimigardefordensis DPN7)
Succinate--CoA ligase (ADP-forming). [EC: 6.2.1.5]; 6.2.1.- [EC: 6.2.1.5]

Predicted SEED Role

"Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 6.2.1.5)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (14/17 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• pyruvate fermentation to acetate V (3/3 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• adipate degradation (4/5 steps found)
• pyruvate fermentation to acetate VI (2/3 steps found)
• TCA cycle III (animals) (7/10 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• adipate biosynthesis (3/5 steps found)
• 3,3'-disulfanediyldipropannoate degradation (2/4 steps found)
• reductive TCA cycle I (6/11 steps found)
• incomplete reductive TCA cycle (3/7 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (11/19 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• C5-Branched dibasic acid metabolism
• Citrate cycle (TCA cycle)
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.5

Use Curated BLAST to search for 6.2.1.5

Sequence Analysis Tools

See G8QNJ6 at UniProt or InterPro

Protein Sequence (298 amino acids)

>Dsui_1446 succinyl-CoA synthetase, alpha subunit (Dechlorosoma suillum PS)
MSILINKDTKVITQGITGKTGQFHTEKCQEYANGKNCFVAGVNPKKAGEKIFDIPIYATV
KEAAKDTGATVSVIYVPPAGAAAAIWEAVEADLDLAICITEGIPVRDMLEVRNKMKEKEA
KGGKKTLLLGPNCPGLITPDEIKIGIMPGHIHKKGRIGVVSRSGTLTYEAVAQLTEIGLG
QSSAVGIGGDPINGLKHIDVMKMFNDDPDTDAVIMIGEIGGPDEAEAAMWCKANMKKPIV
GFIAGVTAPAGKRMGHAGALISGGADTADAKLAIMEECGFTVTRNPSEMGRLLKGLLK