Protein Info for Dsui_1324 in Dechlorosoma suillum PS

Annotation: prolyl-tRNA synthetase, family II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 570 TIGR00409: proline--tRNA ligase" amino acids 1 to 563 (563 residues), 780 bits, see alignment E=8.4e-239 PF00587: tRNA-synt_2b" amino acids 95 to 455 (361 residues), 114.1 bits, see alignment E=1.2e-36 PF04073: tRNA_edit" amino acids 258 to 370 (113 residues), 93.8 bits, see alignment E=1.3e-30 PF03129: HGTP_anticodon" amino acids 472 to 564 (93 residues), 67.4 bits, see alignment E=1.6e-22

Best Hits

Swiss-Prot: 77% identical to SYP_DECAR: Proline--tRNA ligase (proS) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 77% identity to app:CAP2UW1_3010)

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QME6 at UniProt or InterPro

Protein Sequence (570 amino acids)

>Dsui_1324 prolyl-tRNA synthetase, family II (Dechlorosoma suillum PS)
MRTSQFFISTLKEAPSDAEVISHKLMLRAGLIKRLAGGIYTWMPLGLKVLRKVEKVVREE
MDRAGAIELLMPAVVPAELWQETGRWEKYGPELLRFKDRHQRDFVIGPTHEEVITDVVRK
EIKSYRQLPVHFYQVQSKFRDEIRPRFGVMRGREFLMKDGYSFHTSFADLEREYKHMYDT
YCRIFTRLGLQFRAVAADTGSIGGTGSHEFHVLADSGEDAIAFCPDSDYAANVELAEALA
PAAARAAASQAMTKVATPGKTQCADVAAFLGASAEQTVKAVAVMRTEGGFALLLLRGDHE
LNEIKAEKIIGEFRFAKDDEIVAALGCKPGYIGPVGIKADIPVIADRTVAAMADFICGAN
EAEHHLTGVNFGRDLPEPGQVADIRNVVEGDPSPDGKGQLGLCRGIEVGHIFQLRTRYSE
ALNCKYLDENGKEQIMEMGCYGIGVSRIVGAAIEQGNDDKGIVFPAAIAPFEVAIVPMGY
HKSEAVKAAADQLLADLKAAGIDVLLDDRNERPGSMFADMELIGIPHRIVVGERGLAEGK
LEYKGRRDAEAQMVDAAGIVALLQGKLCAA