Protein Info for Dsui_1268 in Dechlorosoma suillum PS

Annotation: dinucleotide-utilizing enzyme possibly involved in molybdopterin or thiamin biosynthesis

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 51% identical to THIF_ECOLI: Sulfur carrier protein ThiS adenylyltransferase (thiF) from Escherichia coli (strain K12)

KEGG orthology group: K11996, adenylyltransferase and sulfurtransferase (inferred from 79% identity to azo:azo2804)

MetaCyc: 51% identical to sulfur carrier protein ThiS adenylyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-9789 [EC: 2.7.7.73]

Predicted SEED Role

"Sulfur carrier protein adenylyltransferase ThiF" in subsystem Thiamin biosynthesis

MetaCyc Pathways

superpathway of thiamine diphosphate biosynthesis II (8/11 steps found)
superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
thiazole component of thiamine diphosphate biosynthesis II (4/7 steps found)
thiazole component of thiamine diphosphate biosynthesis I (3/6 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.73

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QLS8 at UniProt or InterPro

Protein Sequence (249 amino acids)

>Dsui_1268 dinucleotide-utilizing enzyme possibly involved in molybdopterin or thiamin biosynthesis (Dechlorosoma suillum PS)
MNDQQLLRYSRHILLDPIGIEGQERFLTARALVIGAGGLGSPAALYLAAAGVGTLALADG
DTVDLTNLQRQILHTEASVGRPKAESGRDALQRLNPETRVEVIARRLEGADLEAEVAKAD
VVLDCCDNFATRHAVNRACVKLGKPLVSGAAVRFDGQISVFDTRQPGAPCYHCLFPEGED
VEEVRCATMGVFAPLTGIIGTLQAAEALKLLAGCGQTLGGRLLLLDALTMEWRTVRLQKD
PGCPVCGSR