Protein Info for Dsui_1224 in Dechlorosoma suillum PS

Annotation: leucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 889 TIGR00396: leucine--tRNA ligase" amino acids 25 to 889 (865 residues), 1101.5 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 35 to 244 (210 residues), 92.2 bits, see alignment E=6.5e-30 amino acids 441 to 601 (161 residues), 34.1 bits, see alignment E=2.7e-12 amino acids 649 to 689 (41 residues), 26.9 bits, see alignment 4.1e-10 PF09334: tRNA-synt_1g" amino acids 59 to 191 (133 residues), 65.6 bits, see alignment E=7.2e-22 PF13603: tRNA-synt_1_2" amino acids 241 to 427 (187 residues), 238.2 bits, see alignment E=9.5e-75 PF08264: Anticodon_1" amino acids 731 to 853 (123 residues), 51.7 bits, see alignment E=1.8e-17

Best Hits

Swiss-Prot: 62% identical to SYL_ACIAD: Leucine--tRNA ligase (leuS) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 80% identity to app:CAP2UW1_3066)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QLN4 at UniProt or InterPro

Protein Sequence (889 amino acids)

>Dsui_1224 leucyl-tRNA synthetase (Dechlorosoma suillum PS)
MEQERTGYNFTLCNEFLAAQMQEKYQPAEVELAAQQHWVKTQAARAVEDTTKPKYYCLSM
FPYPSGKLHMGHVRNYTIGDVLTRFHKMQGYNVLQPMGWDAFGMPAENAAIQNKVPPAKW
TYANIDYMRSQLQRLGFAIDWEREFATCTPEYYRWEQWLFTRLYEKGLIYKKLGTVNWDP
VDHTVLANEQVIDGRGWRSGALVEKREIPMYYMKITAYAEELLADLDQLPGWPEQVRLMQ
KNWIGKSTGVRFAFPLADNSDEKLWVFTTRADTIMGVTFVAVAAEHPLATRAAANNPALA
AFIEECKKGGVAEADIATMEKKGMPTGIFVQHPLTGEQVEVWVGNYVLMGYGEGAVMAVP
AHDERDFAFAKKYNLAIKQVIAAAGETFSLDGWQEWYGDKSRGTCVNSGKYDGLAYEAAV
DAIAADLAAKGLGEKKVQFRLRDWGISRQRYWGCPIPVIHCESCGDVPVPDDQLPVVLPE
NVEITGAGSPLAKMPEFYECTCPKCGKPARRETDTMDTFVESSWYFLRYACPDNTQAMVD
ERVAYWCKGGIDQYIGGIEHAILHLLYSRFFTKLMRDVGLFGEQKLDEPFANLLTQGMVV
APTFYRDAEGGKKEWINPADVDVTTDERGRPTGATLKADGQPVVIGGTEKMSKSKNNGVD
PQSLMDEYGADTARLFMMFAAPPDQSLEWSDAGVEGAFRFLKRLWKLTHDHVAAGIVPAY
SGGELEGSLKDFRRQLHQTIGKVTDDYGRRKQFNTAIAAVMELLNAFAKLPDSPAASSVK
QEALEAAVLMLFPIVPHACQALYAELKPGADAGQAAFPKADAAALVQSEIELMLQVNGKL
RGSIKVPADAAKDAIEAAALASETVQKHLDGQAPKKVVVVPGRLVNIVV