Protein Info for Dsui_1099 in Dechlorosoma suillum PS

Annotation: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 1 to 449 (449 residues), 617.8 bits, E=5.9e-190
• PF01128: IspD
  • amino acids 2 to 119 (118 residues), 29.9 bits, E=1.5e-10
• PF00483: NTP_transferase
  • amino acids 4 to 217 (214 residues), 52.3 bits, E=1.9e-17
• PF12804: NTP_transf_3
  • amino acids 4 to 124 (121 residues), 72.9 bits, E=1.1e-23
• PF14602: Hexapep_2
  • amino acids 390 to 424 (35 residues), 31.1 bits, 4.7e-11
• PF00132: Hexapep
  • amino acids 390 to 424 (35 residues), 31.6 bits, 2.9e-11

Best Hits

Swiss-Prot: 74% identical to GLMU_DECAR: Bifunctional protein GlmU (glmU) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 75% identity to app:CAP2UW1_4176)

MetaCyc: 53% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (3/6 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (3/7 steps found)
• peptidoglycan recycling I (8/14 steps found)
• chitin biosynthesis (4/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (6/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See G8QK68 at UniProt or InterPro

Protein Sequence (452 amino acids)

>Dsui_1099 UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (Dechlorosoma suillum PS)
MNIVILAAGQGKRMHSHLPKVLHPIAGKALAAHVLDTARHLGQSRICLVVGHGGEVVRSA
LAAADVACAVQEPQLGTGHAVMQALPLLDGEGMTLVLYGDVPLIQATTLQRLVQAAGDGL
AVLTVDLENPKGYGRIIRDSAGRVQRIVEEKDATPAERLVRETNTGIMALPTARLKDWLS
RLSNDNAQGEYYLTDVVALAVADGVPVHTAQPEGEWEVAGVNSKVQLAELERVYQLRLAH
GLLEQGVRLADPARLDVRGQLRCGRDVAIDVGCVFEGEVVLEEAVDIGPYCVLKNVTVKA
GARIAAFSHLEGAVVGPDAIVGPYARLRPGTELGAEAHVGNFVELKNSILGPGSKANHLA
YVGDATVGQRVNIGAGTITCNYDGANKHRTVIEDDAFIGSDSQLVAPVTVGRGATLGAGT
TLTKDAPADSLTVSRARQVSLAGWKRPEKVKK