Protein Info for Dsui_0977 in Dechlorosoma suillum PS

Annotation: acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 378 PF02771: Acyl-CoA_dh_N" amino acids 4 to 116 (113 residues), 140.3 bits, see alignment E=6.7e-45 PF02770: Acyl-CoA_dh_M" amino acids 120 to 215 (96 residues), 97.6 bits, see alignment E=7.7e-32 PF00441: Acyl-CoA_dh_1" amino acids 227 to 375 (149 residues), 184.7 bits, see alignment E=2.4e-58 PF08028: Acyl-CoA_dh_2" amino acids 242 to 364 (123 residues), 106.2 bits, see alignment E=3.3e-34

Best Hits

Swiss-Prot: 51% identical to ACADS_RAT: Short-chain specific acyl-CoA dehydrogenase, mitochondrial (Acads) from Rattus norvegicus

KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 97% identity to dar:Daro_0096)

MetaCyc: 63% identical to short-chain acyl-CoA dehydrogenase monomer (Pseudomonas putida KT2440)
RXN-13449 [EC: 1.3.8.1]

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4); Butyryl-CoA dehydrogenase (EC 1.3.99.2)" (EC 1.3.8.4, EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.4, 1.3.99.-, 1.3.99.2

Use Curated BLAST to search for 1.3.8.1 or 1.3.8.4 or 1.3.99.- or 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QJ14 at UniProt or InterPro

Protein Sequence (378 amino acids)

>Dsui_0977 acyl-CoA dehydrogenase (Dechlorosoma suillum PS)
MILTQEQEMIRDSMRAFAQERLAPFAAEWDKNHTFPAEALKELGELGAMGMCVPEEWGGA
GMDYMSLVLTLEEIAAGDGATSTIVSVQNSLACGITQKYGTDQQKEEWLKPLARGEKLGC
FCLTEPHTGSDAAAITTRADRDGDHFVLNGVKQFITTGKHAHMAIVFAVTDKAAGKKGIS
CFLVPTDTKGFIVGRTEEKMGQHASDTVQIIFEDCRVPASALLGKEGEGYKIALSNLEAG
RIGIAAQSIGMARAAFEAAVRYAKERVTFGQPIIEHQAVNFRLADMNTLLDAARLMVWRA
AALKDAGKPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRISQIY
EGANDIQRLVIGRSIAQE