Protein Info for Dsui_0955 in Dechlorosoma suillum PS

Annotation: nitrogenase iron protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 PF02374: ArsA_ATPase" amino acids 5 to 46 (42 residues), 30.6 bits, see alignment 5.4e-11 TIGR01287: nitrogenase iron protein" amino acids 5 to 279 (275 residues), 460.4 bits, see alignment E=9.2e-143 PF00142: Fer4_NifH" amino acids 5 to 276 (272 residues), 436.6 bits, see alignment E=9.4e-135 PF13614: AAA_31" amino acids 7 to 74 (68 residues), 38.2 bits, see alignment E=3.6e-13 PF01656: CbiA" amino acids 9 to 228 (220 residues), 42.2 bits, see alignment E=1.9e-14

Best Hits

Swiss-Prot: 90% identical to NIFH_ACIF5: Nitrogenase iron protein (nifH) from Acidithiobacillus ferrooxidans (strain ATCC 53993)

KEGG orthology group: K02588, nitrogenase iron protein NifH [EC: 1.18.6.1] (inferred from 90% identity to afr:AFE_1522)

MetaCyc: 78% identical to [FeMo]-nitrogenase complex reductase component monomer (Azotobacter vinelandii)
RXN-17059

Predicted SEED Role

"Nitrogenase (molybdenum-iron) reductase and maturation protein NifH" in subsystem Nitrogen fixation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QIZ2 at UniProt or InterPro

Protein Sequence (291 amino acids)

>Dsui_0955 nitrogenase iron protein (Dechlorosoma suillum PS)
MSALRQIAFYGKGGIGKSTTSQNTLAALAEMGQKILIVGCDPKADSTRLILHAKAQDTVL
SLAADAGSVEDLELEDVLKVGYRDIRCVESGGPEPGVGCAGRGVITAINFLEENGAYDGV
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAANNISKGILKYANAGGVRL
GGLICNERKTDKEYELADALAKKLGTKLIHFVPRDNVVQHAELRRMTVLEYDPESKQAGE
YRALAQKVHANAGNGVIPTPISMDELEDLLLEFGIMSKEDESIVGKSKSAA