Protein Info for Dsui_0816 in Dechlorosoma suillum PS

Annotation: methionyl-tRNA formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 PF00551: Formyl_trans_N" amino acids 1 to 180 (180 residues), 164.3 bits, see alignment E=2.6e-52 TIGR00460: methionyl-tRNA formyltransferase" amino acids 1 to 295 (295 residues), 290.3 bits, see alignment E=8.3e-91 PF02911: Formyl_trans_C" amino acids 200 to 295 (96 residues), 91 bits, see alignment E=4.9e-30

Best Hits

Swiss-Prot: 65% identical to FMT_DECAR: Methionyl-tRNA formyltransferase (fmt) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K00604, methionyl-tRNA formyltransferase [EC: 2.1.2.9] (inferred from 65% identity to dar:Daro_0022)

MetaCyc: 53% identical to 10-formyltetrahydrofolate:L-methionyl-tRNAfMet N-formyltransferase (Escherichia coli K-12 substr. MG1655)
Methionyl-tRNA formyltransferase. [EC: 2.1.2.9]; 2.1.2.9 [EC: 2.1.2.9]

Predicted SEED Role

"Methionyl-tRNA formyltransferase (EC 2.1.2.9)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or Folate Biosynthesis (EC 2.1.2.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.9

Use Curated BLAST to search for 2.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QHS2 at UniProt or InterPro

Protein Sequence (308 amino acids)

>Dsui_0816 methionyl-tRNA formyltransferase (Dechlorosoma suillum PS)
MKIVFAGTPEFAARALAALYEAGHDIPLVLTQPDRPAGRGMTLQASPVKQLALSRGSEVF
QPLTLKDEAAQEKLRLLAPDVMVVAAYGLILPQAVLDIPRHGCLNIHASLLPRWRGAAPI
HRAILAGDAESGVCIMQMEAGLDTGPVLLSASTAITAQDTSQTLHDKLAVQGAQLIVSAL
QRLPLPAVAQPEAGVTYAAKLDKSEAPLDWRRPAAELDRQIRAFTPFPGTTAVLDGAPLK
VWAALPRSESGVPGTVLAADKHGILVACGSGSLLLTELQKAGGKRLPVAQFLAGHGVSAG
ARFELPAA